WB
Western blot analysis of various lysates using TriMethyl-Histone H3-K9 Rabbit pAb at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.IHC
Immunohistochemistry analysis of paraffin embedded Rat testis using TriMethyl-Histone H3-K9 Rabbit pAb at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.ICC/IF
Immunofluorescence analysis of HeLa cells using TriMethyl-Histone H3-K9 Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.DB
Dot-blot analysis of all sorts of methylation peptides using TriMethyl-Histone H3-K9 antibody.| Product Name | TriMethyl-Histone H3-K9 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthetic trimethylated peptide around K9 of human histone H3 (NP_003520.1). |
| Modification | TriMethyl K9 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | DBICC/IFIHCWB |
| WB:1:100-1:500 IHC:1:50-1:200 ICC:1:50-1:200 DB:1:500-1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | H3-4,H3C1 |
|---|---|
| Gene Synonyms | H3t H3.4 H3/g H3FT H3C16 HIST3H3 TriMethyl-Histone H3-K9 |
| Gene Full Name | H3 clustered histone 1 |
| Gene Summary | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015] |
| Alternative Names | H3t H3.4 H3/g H3FT H3C16 HIST3H3 TriMethyl-Histone H3-K9 |
| Molecular Weight(MW) | 16kDa |
| Cellular Localization | Chromosome,Nucleus. |
WB
Western blot analysis of various lysates using TriMethyl-Histone H3-K9 Rabbit pAb at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
IHC
Immunohistochemistry analysis of paraffin embedded Rat testis using TriMethyl-Histone H3-K9 Rabbit pAb at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ICC/IF
Immunofluorescence analysis of HeLa cells using TriMethyl-Histone H3-K9 Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
DB
Dot-blot analysis of all sorts of methylation peptides using TriMethyl-Histone H3-K9 antibody.| Application Notes | WB:1:100-1:500 IHC:1:50-1:200 ICC:1:50-1:200 DB:1:500-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3. |
