WB
Western blot analysis of TTF1 on different lysates with Rabbit anti-TTF1 antibody at 1/1,000 dilution. Lane 1: NCI-H441 cell lysate (20 µg/Lane) Lane 2: TT cell lysate (20 µg/Lane) Lane 3: A549 cell lysate (negative) (20 µg/Lane) Lane 4: HeLa cell lysate (negative) (20 µg/Lane) Lane 5: Mouse liver tissue lysate (40 µg/Lane) Exposure time: 25 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue with Rabbit anti-TTF1 antibody at 1/8,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.ICC/IF
Immunocytochemistry analysis of TT (positive) and A549 (negative) labeling TTF1 with Rabbit anti-TTF1 antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-TTF1 antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.| Product Name | TTF1 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human TTF1 aa 272-371/371. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:4000-1:8000 IHC:1:1000-1:5000 ICC:1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | NKX2-1 |
|---|---|
| Gene Synonyms | BCH BHC NK-2 TEBP TTF1 NKX2A NMTC1 T/EBP TITF1 TTF-1 NKX2.1 |
| Gene Full Name | NK2 homeobox 1 |
| Gene Summary | This gene encodes a protein initially identified as a thyroid-specific transcription factor. The encoded protein binds to the thyroglobulin promoter and regulates the expression of thyroid-specific genes but has also been shown to regulate the expression of genes involved in morphogenesis. Mutations and deletions in this gene are associated with benign hereditary chorea, choreoathetosis, congenital hypothyroidism, and neonatal respiratory distress, and may be associated with thyroid cancer. Multiple transcript variants encoding different isoforms have been found for this gene. This gene shares the symbol/alias 'TTF1' with another gene, transcription termination factor 1, which plays a role in ribosomal gene transcription. [provided by RefSeq, Feb 2014] |
| Alternative Names | AV026640 antibody BCH antibody Benign chorea antibody BHC antibody Homeobox protein NK 2 homolog A antibody Homeobox protein NK-2 homolog A antibody Homeobox protein Nkx 2.1 antibody Homeobox protein Nkx-2.1 antibody Homeobox protein Nkx2.1 antibody NK 2 antibody AV026640 antibody BCH antibody Benign chorea antibody BHC antibody Homeobox protein NK 2 homolog A antibody Homeobox protein NK-2 homolog A antibody Homeobox protein Nkx 2.1 antibody Homeobox protein Nkx-2.1 antibody Homeobox protein Nkx2.1 antibody NK 2 antibody NK 2 homolog A antibody NK2 antibody NK2 homeobox 1 antibody NK2 drosophila homolog of A antibody NK2.1 mouse homolog of antibody Nkx 2 1 antibody NKX 2.1 antibody NKX 2A antibody NKX2 1 antibody Nkx2-1 antibody NKX2.1 antibody NKX21_HUMAN antibody NKX2A antibody T EBP antibody T/EBP antibody TEBP antibody Thyroid nuclear factor 1 antibody Thyroid nuclear factor antibody Thyroid specific enhancer binding protein antibody Thyroid transcription factor 1 antibody Tin man antibody Tinman antibody TITF 1 antibody TITF1 antibody TTF 1 antibody TTF-1 antibody TTF1 antibody |
| Molecular Weight(MW) | 39kDa(Observed band size: 39/42kDa) |
| Cellular Localization | Nucleus. |
WB
Western blot analysis of TTF1 on different lysates with Rabbit anti-TTF1 antibody at 1/1,000 dilution. Lane 1: NCI-H441 cell lysate (20 µg/Lane) Lane 2: TT cell lysate (20 µg/Lane) Lane 3: A549 cell lysate (negative) (20 µg/Lane) Lane 4: HeLa cell lysate (negative) (20 µg/Lane) Lane 5: Mouse liver tissue lysate (40 µg/Lane) Exposure time: 25 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue with Rabbit anti-TTF1 antibody at 1/8,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC/IF
Immunocytochemistry analysis of TT (positive) and A549 (negative) labeling TTF1 with Rabbit anti-TTF1 antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-TTF1 antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.| Application Notes | WB:1:4000-1:8000 IHC:1:1000-1:5000 ICC:1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
