WB
Western blot analysis of various lysates using CDKN2A/p19ARF Rabbit mAb at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Negative control (NC): NIH/3T3 Exposure time: 0.5s.IHC
Immunohistochemistry analysis of paraffin embedded Mouse brain tissue using CDKN2A/p19ARF Rabbit mAb at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.ICC/IF
Confocal imaging of paraffin-embedded Mouse brain tissue using CDKN2A/p19ARF Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. objective: 40x.| Product Name | CDKN2A/p19ARF Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 1-100 of mouse CDKN2A/p19ARF (NP_034007.1). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:1000-1:10000 IHC:1:300-1:1200 ICC:1:50-1:200 |
|
| Species Reactivity | MouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Cdkn2a |
|---|---|
| Gene Synonyms | Arf p16 MTS1 Pctr1 p19ARF p16INK4a p19 ARF-INK4a INK4a-ARF Ink4a/Arf p16(INK4a) |
| Gene Full Name | cyclin dependent kinase inhibitor 2A |
| Gene Summary | Enables DNA-binding transcription factor binding activity; MDM2/MDM4 family protein binding activity; and enzyme inhibitor activity. Involved in several processes, including negative regulation of lymphocyte proliferation; positive regulation of apoptotic process; and regulation of DNA-templated transcription. Acts upstream of or within several processes, including keratinocyte proliferation; negative regulation of mammary gland epithelial cell proliferation; and negative regulation of protein metabolic process. Located in cytoplasm and granular component. Part of protein-containing complex. Is expressed in several structures, including central nervous system; eye; genitourinary system; immune system; and umbilical artery. Used to study high grade glioma; malignant peripheral nerve sheath tumor; melanoma (multiple); and pancreatic carcinoma (multiple). Human ortholog(s) of this gene implicated in several diseases, including breast cancer (multiple); carcinoma (multiple); hematologic cancer (multiple); high grade glioma (multiple); and melanoma (multiple). Orthologous to human CDKN2A (cyclin dependent kinase inhibitor 2A). [provided by Alliance of Genome Resources, Dec 2024] |
| Molecular Weight(MW) | 21kDa |
| Cellular Localization | Nucleus, nucleoplasm. |
WB
Western blot analysis of various lysates using CDKN2A/p19ARF Rabbit mAb at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Negative control (NC): NIH/3T3 Exposure time: 0.5s.
IHC
Immunohistochemistry analysis of paraffin embedded Mouse brain tissue using CDKN2A/p19ARF Rabbit mAb at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.
ICC/IF
Confocal imaging of paraffin-embedded Mouse brain tissue using CDKN2A/p19ARF Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. objective: 40x.| Application Notes | WB:1:1000-1:10000 IHC:1:300-1:1200 ICC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3. |
