WB
Western blot analysis of MAP1LC3A on different lysates with Rabbit anti-MAP1LC3A antibody at 1/1,000 dilution. Lane 1: HeLa cell lysate, Lane 2: HeLa treated with 50μM Chloroquine for 18 hours cell lysate, Lane 3: C2C12 cell lysate, Lane 4: C2C12 treated with 50μM Chloroquine for 18 hours cell lysate, Lane 5: C6 cell lysate, Lane 6: C6 treated with 50μM Chloroquine for 18 hours cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-MAP1LC3A antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.ICC/IF
Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-MAP1LC3A antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Product Name | MAP1LC3A Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within Human MAP1LC3A aa 81-121 / 121. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:200 ICC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | MAP1LC3A |
|---|---|
| Gene Synonyms | LC3 LC3A ATG8E MAP1ALC3 MAP1BLC3 |
| Gene Full Name | microtubule associated protein 1 light chain 3 alpha |
| Gene Summary | MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene. The expression of variant 1 is suppressed in many tumor cell lines, suggesting that may be involved in carcinogenesis. [provided by RefSeq, Feb 2012] |
| Alternative Names | ATG8E antibody Autophagy-related protein LC3 A antibody Autophagy-related ubiquitin-like modifier LC3 A antibody LC3 antibody LC3A antibody MAP1 light chain 3 like protein 1 antibody MAP1 light chain 3-like protein 1 antibody MAP1A/1B light chain 3 A antibody MAP1A/MAP1B LC3 A antibody MAP1A/MAP1B light chain 3 A antibody ATG8E antibody Autophagy-related protein LC3 A antibody Autophagy-related ubiquitin-like modifier LC3 A antibody LC3 antibody LC3A antibody MAP1 light chain 3 like protein 1 antibody MAP1 light chain 3-like protein 1 antibody MAP1A/1B light chain 3 A antibody MAP1A/MAP1B LC3 A antibody MAP1A/MAP1B light chain 3 A antibody MAP1ALC3 antibody MAP1BLC3 antibody Map1lc3a antibody Microtubule associated proteins 1A/1B light chain 3 antibody Microtubule-associated protein 1 light chain 3 alpha antibody Microtubule-associated proteins 1A and 1B light chain 3 antibody Microtubule-associated proteins 1A/1B light chain 3A antibody MLP3A_HUMAN antibody |
| Molecular Weight(MW) | 14kDa(Observed band size: 14/16kDa) |
| Cellular Localization | Cytoplasm, Endomembrane system, Cytoplasmic vesicle, Microtubule, Membrane. |
WB
Western blot analysis of MAP1LC3A on different lysates with Rabbit anti-MAP1LC3A antibody at 1/1,000 dilution. Lane 1: HeLa cell lysate, Lane 2: HeLa treated with 50μM Chloroquine for 18 hours cell lysate, Lane 3: C2C12 cell lysate, Lane 4: C2C12 treated with 50μM Chloroquine for 18 hours cell lysate, Lane 5: C6 cell lysate, Lane 6: C6 treated with 50μM Chloroquine for 18 hours cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-MAP1LC3A antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC/IF
Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-MAP1LC3A antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:200 ICC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
