WB
Confocal imaging of RAW 264.7 cells using Omnimabs® 488 Rabbit anti-Mouse CD45 mAb (dilution 1:200).DAPI was used for nuclear staining (Blue). objective: 100x.FC
Flow cytometry:1X10^6 C2C12 cells(negative control,left) and C57BL/6 mouse Splenocytes(right) were surface stained with Omnimabs® 488 Rabbit anti-Mouse CD45 mAb(5ul/Test,orange line) or Omnimabs® 488 Rabbit IgG isotype control (5ul/Test,blue line). Non f luorescently stained cells were used as blank control (red line).| Product Name | Omnimabs® 488 Rabbit anti-Mouse CD45 mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 26-566 of mouse CD45 (NP_001104786.2). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IF |
| ICC:1:50 - 1:200 FC:5 μl per 10^6 cells in 100 μl volume |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Ptprc |
|---|---|
| Gene Synonyms | loc B220 Cd45 L-CA Ly-5 T200 CD45R Lyt-4 |
| Gene Full Name | protein tyrosine phosphatase receptor type C |
| Gene Summary | Enables several functions, including heparan sulfate proteoglycan binding activity; heparin binding activity; and protein tyrosine phosphatase activity. Involved in several processes, including lymphocyte differentiation; regulation of macromolecule metabolic process; and regulation of signal transduction. Acts upstream of or within several processes, including T cell activation; positive regulation of lymphocyte activation; and regulation of protein phosphorylation. Located in external side of plasma membrane; focal adhesion; and membrane raft. Is expressed in several structures, including alimentary system; cardiovascular system; hemolymphoid system; placenta; and skin. Used to study Alzheimer's disease and systemic lupus erythematosus. Human ortholog(s) of this gene implicated in multiple sclerosis; primary immunodeficiency disease; and severe combined immunodeficiency. Orthologous to human PTPRC (protein tyrosine phosphatase receptor type C). [provided by Alliance of Genome Resources, Dec 2024] |
| Cellular Localization | Cell membrane,Single-pass type I membrane protein. |
WB
Confocal imaging of RAW 264.7 cells using Omnimabs® 488 Rabbit anti-Mouse CD45 mAb (dilution 1:200).DAPI was used for nuclear staining (Blue). objective: 100x.
FC
Flow cytometry:1X10^6 C2C12 cells(negative control,left) and C57BL/6 mouse Splenocytes(right) were surface stained with Omnimabs® 488 Rabbit anti-Mouse CD45 mAb(5ul/Test,orange line) or Omnimabs® 488 Rabbit IgG isotype control (5ul/Test,blue line). Non f luorescently stained cells were used as blank control (red line).| Application Notes | ICC:1:50 - 1:200 FC:5 μl per 10^6 cells in 100 μl volume |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 2-8℃. Avoid freeze. |
| Storage Buffer | Buffer: PBS with 0.03% proclin300,0.2% BSA,pH7.3. |
Data sheet for OM643596