| Product Name | CD45.2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | loc, B220, Cd45, L-CA, Ly-5, T200, CD45R, Lyt-4, Ptprc |
| Product description | The 104 monoclonal antibody specifically reacts with the mouse CD45 molecule, the Leukocyte Common Antigen (LCA) which occurs in the alloantigen CD45.2-expressing mouse strains (C57BL/6, CBA, 129, A, AKR, C58, DBA/1, DBA/2, BALB/c, and C3H/He). The 104 monoclonal antibody does not react with the leukocytes of the CD45.1-expressing mouse strains (DA, SJL/J, RIII, and STS/A). The CD45 molecule is a member of the Protein Tyrosine Phosphatase (PTP) family, because its intracellular region contains two PTP domains. The extracellular region’s variability is caused by different levels of glycosylation, and the splicing of the 4, 5, and 6 exons. The isoforms found in the mouse strains depend on the activation state, maturation stage and cell type, and are very important in B and T lymphocytes antigen receptor signal transduction. The 104 antibody inhibits some of the B lymphocytes responses, reduces the serum IgG levels, and influences the autoimmune renal pathology.1) Shen, F. W., Tung, J. S., Boyse, E. A. (1986). Further definition of the Ly-5 system.Immunogenetics,24(3), 146-149. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a,k |
| Host Species | Mouse |
| Tested Applications | FACSICCIHCIP |
| FACS:1:200~1:500 ICC:1:200~1:500 IHC:1:50~1:200 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Ptprc |
|---|---|
| Alternative Names | loc B220 Cd45 L-CA Ly-5 T200 CD45R Lyt-4 Ptprc |
| Tissue Specificity | The 104 monoclonal antibody specifically reacts with the mouse CD45 molecule, the Leukocyte Common Antigen (LCA) which occurs in the alloantigen CD45.2-expressing mouse strains (C57BL/6, CBA, 129, A, AKR, C58, DBA/1, DBA/2, BALB/c, and C3H/He). |
| Entrez Gene | 19264 |
|---|
| Application Notes | FACS:1:200~1:500 ICC:1:200~1:500 IHC:1:50~1:200 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | The product should be stored undiluted at 4˚C and should be protected from prolonged exposure to light. Do not freeze. |
| Storage Buffer | Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2. |
Data sheet for OM274685