WB
Western blot analysis of Phospho-PAK4+PAK5+PAK6 (S474 + S560 + S602) on different lysates with Rabbit anti-Phospho-PAK4+PAK5+PAK6 (S474 + S560 + S602) antibody at 1/1,000 dilution. Lane 1: A549 cell lysate (20 µg/Lane) Lane 2: HeLa cell lysate (20 µg/Lane) Lane 3: MCF7 cell lysate (20 µg/Lane) Lane 4: HUVEC cell lysate (10 µg/Lane) Lane 5: HUVEC treated with 50ng/mL VEGF for 2 minutes cell lysate (30 µg/Lane) Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.| Product Name | Phospho-PAK4+PAK5+PAK6 (S474 + S560 + S602) Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic phosphopeptide corresponding to residues surrounding Ser474 of human PAK4. |
| Modification | p-S474 + S560 + S602 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | PAK4,PAK5,PAK6 |
|---|---|
| Alternative Names | KIAA1142 antibody p21 activated kinase 4 antibody p21 Cdc42/Rac1-actiated kinase 4 antibody P21 protein (Cdc42/Rac) activated kinase 4 antibody p21(CDKN1A) activated kinase 4 antibody p21-activated kinase 4 antibody PAK 4 antibody PAK-4 antibody Pak4 antibody PAK4_HUMAN antibody KIAA1142 antibody p21 activated kinase 4 antibody p21 Cdc42/Rac1-actiated kinase 4 antibody P21 protein (Cdc42/Rac) activated kinase 4 antibody p21(CDKN1A) activated kinase 4 antibody p21-activated kinase 4 antibody PAK 4 antibody PAK-4 antibody Pak4 antibody PAK4_HUMAN antibody Protein kinase related to S.cerevisiae STE20 effector for Cdc42Hs antibody Serine threonine kinase PAK 4 antibody Serine/threonine protein kinase PAK 4 antibody Serine/threonine protein kinase PAK4 antibody Serine/threonine-protein kinase PAK 4 antibody EC 2.7.11.1 antibody KIAA1264 antibody MGC26232 antibody p21 activated kinase 7 antibody p21 protein (Cdc42/Rac)-activated kinase 7 antibody p21(CDKN1A) activated kinase 7 antibody p21-activated kinase 5 antibody p21-activated kinase 7 antibody PAK 5 antibody PAK 7 antibody PAK-5 antibody PAK-7 antibody PAK5 antibody PAK7 antibody PAK7_HUMAN antibody Protein kinase PAK5 antibody Serine/threonine protein kinase PAK 7 antibody Serine/threonine-protein kinase PAK 7 antibody CDKN1A activated kinase 6 antibody EC 2.7.1.37 antibody p21 activated protein kinase 6 antibody p21 protein (Cdc42/Rac)-activated kinase 6 antibody p21(CDKN1A) activated kinase 6 antibody p21-activated kinase 6 antibody p21activated kinase 6 antibody PAK 6 antibody PAK-6 antibody Pak6 antibody PAK6_HUMAN antibody Serine threonine protein kinase PAK 6 antibody Serine/threonine protein kinase PAK 6 antibody Serine/threonine protein kinase PAK6 antibody Serine/threonine-protein kinase PAK 6 antibody |
| Molecular Weight(MW) | 64/81/75 kDa |
| Function | Serine/threonine-protein kinase PAK 4 is an enzyme that in humans is encoded by the PAK4 gene. Serine/threonine-protein kinase PAK 5 is an enzyme that in humans is encoded by the PAK5 gene. Serine/threonine-protein kinase PAK 6 is an enzyme that in humans is encoded by the PAK6 gene. PAK4 is one of six members of the PAK family of serine/threonine kinases which are divided into group I (PAK1, PAK2 and PAK3) and group II (PAK4, PAK6 and PAK5/7). PAK4 localizes in sub-cellular domains of the cytoplasm and nucleus. PAK4 regulates cytoskeleton remodeling, phenotypic signaling and gene expression, and affects directional motility, invasion, metastasis, and growth. Similar to PAK1, PAK4-signaling-dependent cellular functions also regulate both physiologic and disease processes such as cancer, as PAK4 is overexpressed and/or hyperstimulated in human cancer, at-large. The PAK5 enzyme is one of three members of Group II PAK family of serine/threonine kinases, and evolutionary conserved across species. PAK6 belongs to the Group II PAK family members. Like other family members, PAK6 is also evolutionary conserved across species. Phospho-PAK4+PAK5+PAK6 (S474+S560+S602) Antibody detects endogenous levels of PAK4, PAK5 and PAK6 only when phosphorylated at serine 474, 602, or 560, respectively. |
| Cellular Localization | Cytoplasm; Mitochondrion, Cytoplasm, Nucleus; Cytoplasm, Nucleus. |
WB
Western blot analysis of Phospho-PAK4+PAK5+PAK6 (S474 + S560 + S602) on different lysates with Rabbit anti-Phospho-PAK4+PAK5+PAK6 (S474 + S560 + S602) antibody at 1/1,000 dilution. Lane 1: A549 cell lysate (20 µg/Lane) Lane 2: HeLa cell lysate (20 µg/Lane) Lane 3: MCF7 cell lysate (20 µg/Lane) Lane 4: HUVEC cell lysate (10 µg/Lane) Lane 5: HUVEC treated with 50ng/mL VEGF for 2 minutes cell lysate (30 µg/Lane) Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.| Application Notes | WB:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
