WB
Western blot analysis using WT1 mouse mAb against HEK293 (1), COS7 (2), and PC-3 (3) cell lysate.IHC
Immunohistochemical analysis of paraffin embedded human colon cancer tissues using WT1 mouse mAb with DAB staining.ICC/IF
Immunofluorescence analysis of Hela cells using WT1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.FC
Flow cytometric analysis of Hela cells using WT1 mouse mAb (green) and negative control (red).| Product Name | Mouse Monoclonal Antibody to WT1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Purified recombinant fragment of human WT1 (AA: 1-181) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:200-1:1000 ICC:1:200-1:1000 FC:1:200-1:400 |
|
| Species Reactivity | HumanMonkey |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | WT1 |
|---|---|
| Gene Synonyms | GUD AWT1 WAGR WT-1 WT33 NPHS4 WIT-2 |
| Gene Full Name | WT1 transcription factor |
| Gene Summary | This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilms tumor. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation codon upstream of, and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated. [provided by RefSeq, Mar 2015] |
| Alternative Names | GUD AWT1 WAGR WT33 NPHS4 WIT-2 |
| Molecular Weight(MW) | 49kDa |
| Cellular Localization | Cytoplasm,Nucleus. |
WB
Western blot analysis using WT1 mouse mAb against HEK293 (1), COS7 (2), and PC-3 (3) cell lysate.
IHC
Immunohistochemical analysis of paraffin embedded human colon cancer tissues using WT1 mouse mAb with DAB staining.
ICC/IF
Immunofluorescence analysis of Hela cells using WT1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
FC
Flow cytometric analysis of Hela cells using WT1 mouse mAb (green) and negative control (red).| Application Notes | WB:1:500-1:2000 IHC:1:200-1:1000 ICC:1:200-1:1000 FC:1:200-1:400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide. |
