WB
Western blot analysis of various lysates, using COL11A1 Rabbit mAb at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.ICC/IF
Confocal imaging of A-204 cells using COL11A1 Rabbit mAb(dilution 1:200)(Red). DAPI was used for nuclear staining (blue). objective: 100x.| Product Name | COL11A1 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 301-400 of human COL11A1 mAb (NP_001845.3). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:2000-1:12000 ICC:1:200-1:800 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | COL11A1 |
|---|---|
| Gene Synonyms | STL2 COLL6 CO11A1 DFNA37 |
| Gene Full Name | collagen type XI alpha 1 chain |
| Gene Summary | This gene encodes one of the two alpha chains of type XI collagen, a minor fibrillar collagen. Type XI collagen is a heterotrimer but the third alpha chain is a post-translationally modified alpha 1 type II chain. Mutations in this gene are associated with type II Stickler syndrome and with Marshall syndrome. A single-nucleotide polymorphism in this gene is also associated with susceptibility to lumbar disc herniation. Multiple transcript variants have been identified for this gene. [provided by RefSeq, Nov 2009] |
| Alternative Names | STL2 COLL6 CO11A1 DFNA37 COL11A1 |
| Molecular Weight(MW) | 167kDa/176kDa/181kDa/182kDa(Observed MW 280kDa) |
| Cellular Localization | Secreted,extracellular matrix,extracellular space. |
WB
Western blot analysis of various lysates, using COL11A1 Rabbit mAb at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
ICC/IF
Confocal imaging of A-204 cells using COL11A1 Rabbit mAb(dilution 1:200)(Red). DAPI was used for nuclear staining (blue). objective: 100x.| Application Notes | WB:1:2000-1:12000 ICC:1:200-1:800 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | Buffer: 59% PBS with 0.01% Sodium azide,0.05% BSA,40% Glycerol,PH 7.2 |
