WB
Western blot analysis of extracts of various cell lines, using RPL14 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L)at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.ICC/IF
Immunofluorescence analysis of MCF7 cells using RPL14 antibody. Blue: DAPI for nuclear staining.| Product Name | RPL14 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-215 of human RPL14 (NP_003964.3). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:500-1:2000 ICC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RPL14 |
|---|---|
| Gene Synonyms | L14 RL14 eL14 hRL14 CTG-B33 CAG-ISL-7 |
| Gene Full Name | ribosomal protein L14 |
| Gene Summary | Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L14E family of ribosomal proteins. It contains a basic region-leucine zipper (bZIP)-like domain. The protein is located in the cytoplasm. This gene contains a trinucleotide (GCT) repeat tract whose length is highly polymorphic; these triplet repeats result in a stretch of alanine residues in the encoded protein. Transcript variants utilizing alternative polyA signals and alternative 5'-terminal exons exist but all encode the same protein. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008] |
| Alternative Names | L14 RL14 eL14 hRL14 CTG-B33 CAG-ISL-7 RPL14 |
| Molecular Weight(MW) | 23kDa(Observed MW 26kDa) |
| Cellular Localization | cytoplasm, cytosol, cytosolic ribosome, extracellular exosome, postsynaptic density |
WB
Western blot analysis of extracts of various cell lines, using RPL14 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L)at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
ICC/IF
Immunofluorescence analysis of MCF7 cells using RPL14 antibody. Blue: DAPI for nuclear staining.| Application Notes | WB:1:500-1:2000 ICC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
