WB
Gel: 10%SDS-PAGE, Lysate: 35μg, Lane 1-9: HepG2, Hela, HT-29, A549, COS7, Jurkat, MDCK, PC12, MCF-7 cell lysates, Primary antibody: SSB Antibody at dilution 1/1000, Secondary antibody: Goat anti mouse IgG at 1/8000 dilution, Exposure time: 15 minutes.IHC
The image is immunohistochemistry of paraffin-embedded human Kidney tissue using SSB Antibody at dilution 1/40. (Original magnification: ×200)ICC/IF
The image is immunofluorescence of COS7 cells transiently transfected by pCMV6-ENTRY using SSB Antibody at dilution 1/50.FC
The image is flow cytometry analysis using SSB antibody at dilution 1/100, staining Jurkat cells(Red), compared to a nonspecific negative control antibody(Blue).| Product Name | Anti-SSB mouse monoclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Purified SSB protein expressed in HEK293T cell. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG3 |
| Host Species | Mouse |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:1000-1:5000 IHC:1:30-1:150 ICC:1:50-1:100 FC:1:100-1:200 |
|
| Species Reactivity | DogHumanMonkey |
| Concentration | 0.6mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SSB |
|---|---|
| Gene Synonyms | La LARP3 La/SSB |
| Gene Full Name | small RNA binding exonuclease protection factor La |
| Gene Summary | The protein encoded by this gene is involved in diverse aspects of RNA metabolism, including binding and protecting poly(U) termini of nascent RNA polymerase III transcripts from exonuclease digestion, processing 5' and 3' ends of pre-tRNA precursors, acting as an RNA chaperone, and binding viral RNAs associated with hepatitis C virus. Autoantibodies reacting with this protein are found in the sera of patients with Sjogren syndrome and systemic lupus erythematosus. Alternative promoter usage results in two different transcript variants which encode the same protein. [provided by RefSeq, Jun 2014] |
| Alternative Names | La La/SSB LARP3 |
| Molecular Weight(MW) | 47kDa |
| Cellular Localization | Nucleus. |
WB
Gel: 10%SDS-PAGE, Lysate: 35μg, Lane 1-9: HepG2, Hela, HT-29, A549, COS7, Jurkat, MDCK, PC12, MCF-7 cell lysates, Primary antibody: SSB Antibody at dilution 1/1000, Secondary antibody: Goat anti mouse IgG at 1/8000 dilution, Exposure time: 15 minutes.
IHC
The image is immunohistochemistry of paraffin-embedded human Kidney tissue using SSB Antibody at dilution 1/40. (Original magnification: ×200)
ICC/IF
The image is immunofluorescence of COS7 cells transiently transfected by pCMV6-ENTRY using SSB Antibody at dilution 1/50.
FC
The image is flow cytometry analysis using SSB antibody at dilution 1/100, staining Jurkat cells(Red), compared to a nonspecific negative control antibody(Blue).| Application Notes | WB:1:1000-1:5000 IHC:1:30-1:150 ICC:1:50-1:100 FC:1:100-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
