WB
Western blot analysis of MDM2 on different lysates with Mouse anti-MDM2 antibody at 1/1,000 dilution. Lane 1: HepG2 cell lysate, Lane 2: COS-1cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 46 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Anti-Mouse IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human embryonal carcinoma tissue with Mouse anti-MDM2 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.ICC/IF
Immunocytochemistry analysis of U-2 OS cells treated with or without 10μM NUTLIN 3A for 24 hours labeling MDM2 with Mouse anti-MDM2 antibody at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-MDM2 antibody at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.| Product Name | MDM2 Recombinant Mouse Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human MDM2 aa 1-491 / 491. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCWB |
| WB:1:1000 IHC:1:1000 ICC:1:500 |
|
| Species Reactivity | HumanMonkey |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | MDM2 |
|---|---|
| Gene Synonyms | HDMX LSKB hdm2 ACTFS |
| Gene Full Name | MDM2 proto-oncogene |
| Gene Summary | This gene encodes a nuclear-localized E3 ubiquitin ligase. The encoded protein can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. This gene is itself transcriptionally-regulated by p53. Overexpression or amplification of this locus is detected in a variety of different cancers. There is a pseudogene for this gene on chromosome 2. Alternative splicing results in a multitude of transcript variants, many of which may be expressed only in tumor cells. [provided by RefSeq, Jun 2013] |
| Alternative Names | ACTFS antibody Double minute 2 protein antibody E3 ubiquitin-protein ligase Mdm2 antibody Hdm 2 antibody Hdm2 antibody HDMX antibody MDM 2 antibody MDM2 antibody MDM2 oncogene E3 ubiquitin protein ligase antibody Mdm2 p53 E3 ubiquitin protein ligase homolog antibody ACTFS antibody Double minute 2 protein antibody E3 ubiquitin-protein ligase Mdm2 antibody Hdm 2 antibody Hdm2 antibody HDMX antibody MDM 2 antibody MDM2 antibody MDM2 oncogene E3 ubiquitin protein ligase antibody Mdm2 p53 E3 ubiquitin protein ligase homolog antibody Mdm2 transformed 3T3 cell double minute 2 p53 binding protein (mouse) binding protein 104kDa antibody MDM2_HUMAN antibody MDM2BP antibody Mouse Double Minute 2 antibody MTBP antibody Murine Double Minute Chromosome 2 antibody Oncoprotein Mdm2 antibody p53 Binding Protein Mdm2 antibody p53-binding protein Mdm2 antibody Ubiquitin protein ligase E3 Mdm2 antibody Ubiquitin protein ligase E3 Mdm2 antibody |
| Molecular Weight(MW) | 55 kDa(Observed band size: 90 kDa) |
| Cellular Localization | Nucleus, nucleoplasm, nucleolus, Cytoplasm. |
WB
Western blot analysis of MDM2 on different lysates with Mouse anti-MDM2 antibody at 1/1,000 dilution. Lane 1: HepG2 cell lysate, Lane 2: COS-1cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 46 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Anti-Mouse IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human embryonal carcinoma tissue with Mouse anti-MDM2 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC/IF
Immunocytochemistry analysis of U-2 OS cells treated with or without 10μM NUTLIN 3A for 24 hours labeling MDM2 with Mouse anti-MDM2 antibody at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-MDM2 antibody at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.| Application Notes | WB:1:1000 IHC:1:1000 ICC:1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
