WB
Various lysates were subjected to SDS PAGE followed by western blot with EBP50/NHERF-1 antibody at dilution of 1:10000 and incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin embedded rat kidney tissue slide using EBP50/NHERF-1 antibody at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).ICC/IF
Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using EBP50/NHERF-1 antibody at dilution of 1:600 and Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L).FC
1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-HUMAN EBP50/NHERF-1 and Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.| Product Name | EBP50/NHERF-1 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human SLC9A3R1 protei.n |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:5000-1:50000 IHC:1:1000-1:4000 ICC:1:200-1:800 FC:0.40 ug per 10^6 cells in a 100 µl suspension |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.6mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NHERF1 |
|---|---|
| Gene Synonyms | EBP50 NHERF NHE-RF NHERF-1 NPHLOP2 SLC9A3R1 |
| Gene Full Name | NHERF family PDZ scaffold protein 1 |
| Gene Summary | This gene encodes a sodium/hydrogen exchanger regulatory cofactor. The protein interacts with and regulates various proteins including the cystic fibrosis transmembrane conductance regulator and G-protein coupled receptors such as the beta2-adrenergic receptor and the parathyroid hormone 1 receptor. The protein also interacts with proteins that function as linkers between integral membrane and cytoskeletal proteins. The protein localizes to actin-rich structures including membrane ruffles, microvilli, and filopodia. Mutations in this gene result in hypophosphatemic nephrolithiasis/osteoporosis type 2, and loss of heterozygosity of this gene is implicated in breast cancer.[provided by RefSeq, Sep 2009] |
| Alternative Names | SLC9A3R1 EBP50 NHERF-1 |
| Molecular Weight(MW) | 39kDa(Observed Molecular Weight 50kDa) |
| Cellular Localization | Cell membrane,Cell projection,Cytoplasm,Membrane. |
WB
Various lysates were subjected to SDS PAGE followed by western blot with EBP50/NHERF-1 antibody at dilution of 1:10000 and incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin embedded rat kidney tissue slide using EBP50/NHERF-1 antibody at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
ICC/IF
Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using EBP50/NHERF-1 antibody at dilution of 1:600 and Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L).
FC
1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-HUMAN EBP50/NHERF-1 and Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.| Application Notes | WB:1:5000-1:50000 IHC:1:1000-1:4000 ICC:1:200-1:800 FC:0.40 ug per 10^6 cells in a 100 µl suspension |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
