WB
Western blot analysis of IKK alpha + IKK beta on different lysates with Rabbit anti-IKK alpha + IKK beta antibody at 1/2,000 dilution. Lane 1: HeLa cell lysate, Lane 2: 293T cell lysate, Lane 3: Daudi cell lysate, Lysates/proteins at 15 µg/Lane. Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.ICC/IF
Immunocytochemistry analysis of NIH/3T3 cells labeling IKK alpha + IKK beta with Rabbit anti-IKK alpha + IKK beta antibody at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-IKK alpha + IKK beta antibody at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.ICC/IF
Immunocytochemistry analysis of C6 cells labeling IKK alpha + IKK beta with Rabbit anti-IKK alpha + IKK beta antibody at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-IKK alpha + IKK beta antibody at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.| Product Name | IKK alpha + IKK beta Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within Human IKK beta aa 450-652 / 756. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:2000 ICC:1:100-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | IKBKB,CHUK |
|---|---|
| Gene Synonyms | BPS2 IKK1 IKKA IKBKA IKK-1 TCF16 NFKBIKA IKK-alpha |
| Gene Full Name | component of inhibitor of nuclear factor kappa B kinase complex |
| Gene Summary | This gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor. [provided by RefSeq, Jul 2008] |
| Alternative Names | CHUK antibody Conserved helix loop helix ubiquitous kinase antibody I kappa B kinase 1 antibody I kappa B kinase 2 antibody I Kappa B kinase alpha antibody I Kappa B kinase beta antibody IkB kinase alpha subunit antibody IkBKA antibody IkBKB antibody IKK a kinase antibody CHUK antibody Conserved helix loop helix ubiquitous kinase antibody I kappa B kinase 1 antibody I kappa B kinase 2 antibody I Kappa B kinase alpha antibody I Kappa B kinase beta antibody IkB kinase alpha subunit antibody IkBKA antibody IkBKB antibody IKK a kinase antibody IKK alpha antibody IKK beta antibody IKK1 antibody IKK2 antibody IKKA antibody IKKB antibody IMD15 antibody Inhibitor of kappa light polypeptide gene enhancer in B cells kinase beta antibody Inhibitor of nuclear factor kappa-B kinase subunit alpha antibody Inhibitor of nuclear factor kappa-B kinase subunit beta antibody NFKBIKA antibody NFKBIKB antibody Nuclear factor NF kappa B inhibitor kinase beta antibody Nuclear factor NFkappaB inhibitor kinase alpha antibody TCF 16 antibody TCF16 antibody Transcription factor 16 antibody |
| Molecular Weight(MW) | 87kDa |
| Function | IKK-α is part of the IκB kinase complex that plays an important role in regulating the NF-κB transcription factor. IKK-β is an enzyme that serves as a protein subunit of IκB kinase, which is a component of the cytokine-activated intracellular signaling pathway involved in triggering immune responses. IKK's activity causes activation of a transcription factor known as Nuclear Transcription factor kappa-B or NF-κB. Activated IKK-β phosphorylates a protein called the inhibitor of NF-κB, IκB (IκBα), which binds NF-κB to inhibit its function. IKK-α and IKK-β phosphorylate the IκB proteins, marking them for degradation via ubiquitination and allowing NF-κB transcription factors to go into the nucleus. Once activated, NF-κB transcription factors regulate genes that are implicated in many important cellular processes, including immune response, inflammation, cell death, and cell proliferation. |
| Cellular Localization | Cytoplasm, Nucleus, Membrane raft. |
WB
Western blot analysis of IKK alpha + IKK beta on different lysates with Rabbit anti-IKK alpha + IKK beta antibody at 1/2,000 dilution. Lane 1: HeLa cell lysate, Lane 2: 293T cell lysate, Lane 3: Daudi cell lysate, Lysates/proteins at 15 µg/Lane. Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
ICC/IF
Immunocytochemistry analysis of NIH/3T3 cells labeling IKK alpha + IKK beta with Rabbit anti-IKK alpha + IKK beta antibody at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-IKK alpha + IKK beta antibody at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.
ICC/IF
Immunocytochemistry analysis of C6 cells labeling IKK alpha + IKK beta with Rabbit anti-IKK alpha + IKK beta antibody at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-IKK alpha + IKK beta antibody at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.| Application Notes | WB:1:2000 ICC:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
