WB
Western blot analysis of Phospho-LYN (Y397)/LCK (Y394)/HCK (Y411)/BLK (Y389) on different lysates with Rabbit anti-Phospho-LYN (Y397)/LCK (Y394)/HCK (Y411)/BLK (Y389) antibody at 1/2,000 dilution. Lane 1: COLO205 cell lysate, Lane 2: TT cell lysate (negative), Lane 3: Ramos serum starved for 16 hours cell lysate, Lane 4: Ramos serum starved for 16 hours, then treated with 12μg/mL human IgM witch diluted in the serum-free medium for 2 minutes cell lysate, Lane 5: Ramos serum starved for 16 hours, then treated with 12μg/mL human IgM witch diluted in the serum-free medium for 2 minutes cell lysate, then the membrane treated with λpp for 1 hour, Lysates/proteins at 20 µg/Lane. Exposure time: 23 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.WB
Western blot analysis of Phospho-LYN (Y397)/LCK (Y394)/HCK (Y411)/BLK (Y389) on different lysates with Rabbit anti-Phospho-LYN (Y397)/LCK (Y394)/HCK (Y411)/BLK (Y389) antibody at 1/2,000 dilution. Lane 1: NIH/3T3 cell lysate, Lane 2: NIH/3T3 treated with 50mM sodium orthovanadate for 5 minutes cell lysate, Lane 3: PC-12 cell lysate, Lane 4: PC-12 treated with 1mM sodium orthovanadate for 30 minutes cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: Lane 1-2: 20 seconds; Lane 3-4: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.ICC/IF
Immunocytochemistry analysis of Ramos cells serum starved for 16 hours, then treated with 12μg/mL human IgM witch diluted in the serum-free medium for 2 minutes labeling Phospho-LYN (Y397)/LCK (Y394)/HCK (Y411)/BLK (Y389) with Rabbit anti-Phospho-LYN (Y397)/LCK (Y394)/HCK (Y411)/BLK (Y389) antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Phospho-LYN (Y397)/LCK (Y394)/HCK (Y411)/BLK (Y389) antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.| Product Name | Phospho-LYN (Y397)/LCK (Y394)/HCK (Y411)/BLK (Y389) Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic phospho-peptide corresponding to residues surrounding Tyr394 of human LCK. |
| Modification | p-Y389 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:2000 ICC:1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | LYN,LCK,HCK,BLK |
|---|---|
| Alternative Names | AA407514 antibody EC 2.7.10.2 antibody FLJ26625 antibody Hck 2 antibody JTK 8 antibody JTK8 antibody Lck/Yes related novel protein tyrosine kinase antibody LYN antibody LYN proto oncogene Src family tyrosine kinase antibody LYN_HUMAN antibody AA407514 antibody EC 2.7.10.2 antibody FLJ26625 antibody Hck 2 antibody JTK 8 antibody JTK8 antibody Lck/Yes related novel protein tyrosine kinase antibody LYN antibody LYN proto oncogene Src family tyrosine kinase antibody LYN_HUMAN antibody ONCOGENE LYN antibody p53Lyn antibody p56Lyn antibody Tyrosine protein kinase LYN antibody Tyrosine-protein kinase Lyn antibody V yes 1 Yamaguchi sarcoma viral related oncogene homolog antibody Yamaguchi sarcoma viral (v yes 1) related oncogene homolog antibody IMD22 antibody LCK antibody Lck p56 antibody LCK proto-oncogene Src family tyrosine kinase antibody LCK_HUMAN antibody Leukocyte C-terminal Src kinase antibody LSK antibody Lymphocyte cell specific protein tyrosine kinase antibody Lymphocyte cell-specific protein-tyrosine kinase antibody Lymphocyte specific protein tyrosine kinase antibody Membrane associated protein tyrosine kinase antibody Oncogene lck antibody P56 LCK antibody p56(LSTRA) protein tyrosine kinase antibody p56-LCK antibody p56lck antibody pp58 lck antibody pp58lck antibody Protein YT16 antibody Proto oncogene tyrosine protein kinase LCK antibody Proto-oncogene Lck antibody Protooncogene tyrosine protein kinase LCK antibody T cell specific protein tyrosine kinase antibody T cell-specific protein-tyrosine kinase antibody T lymphocyte specific protein tyrosine kinase p56lck antibody Tyrosine-protein kinase Lck antibody YT 16 antibody YT16 antibody Bmk antibody Hck 1 antibody Hck antibody HCK_HUMAN antibody Hemopoietic cell kinase antibody JTK9 antibody p59-HCK/p60-HCK antibody p59Hck antibody p59HCK/p60HCK antibody p61Hck antibody Tyrosine protein kinase HCK antibody Tyrosine-protein kinase HCK antibody B lymphocyte kinase antibody B lymphoid tyrosine kinase antibody Blk antibody BLK nonreceptor tyrosine kinase antibody BLK_HUMAN antibody MGC 10442 antibody MGC10442 antibody MODY 11 antibody MODY11 antibody P55 BLK antibody p55-BLK antibody Tyrosine kinase B lymphocyte specific antibody Tyrosine protein kinase antibody Tyrosine-protein kinase BLK antibody |
| Molecular Weight(MW) | 58kDa |
| Function | The Src family of protein tyrosine kinases, which includes Src, Lyn, Fyn, Yes, Lck, Blk, and Hck, are important in the regulation of growth and differentiation of eukaryotic cells. Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. While phosphorylation at Tyr416 in the activation loop of the kinase domain upregulates enzyme activity, phosphorylation at Tyr527 in the carboxy-terminal tail by Csk renders the enzyme less active. |
| Cellular Localization | Cell membrane, Cytoplasm, cytosol. |
WB
Western blot analysis of Phospho-LYN (Y397)/LCK (Y394)/HCK (Y411)/BLK (Y389) on different lysates with Rabbit anti-Phospho-LYN (Y397)/LCK (Y394)/HCK (Y411)/BLK (Y389) antibody at 1/2,000 dilution. Lane 1: COLO205 cell lysate, Lane 2: TT cell lysate (negative), Lane 3: Ramos serum starved for 16 hours cell lysate, Lane 4: Ramos serum starved for 16 hours, then treated with 12μg/mL human IgM witch diluted in the serum-free medium for 2 minutes cell lysate, Lane 5: Ramos serum starved for 16 hours, then treated with 12μg/mL human IgM witch diluted in the serum-free medium for 2 minutes cell lysate, then the membrane treated with λpp for 1 hour, Lysates/proteins at 20 µg/Lane. Exposure time: 23 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
WB
Western blot analysis of Phospho-LYN (Y397)/LCK (Y394)/HCK (Y411)/BLK (Y389) on different lysates with Rabbit anti-Phospho-LYN (Y397)/LCK (Y394)/HCK (Y411)/BLK (Y389) antibody at 1/2,000 dilution. Lane 1: NIH/3T3 cell lysate, Lane 2: NIH/3T3 treated with 50mM sodium orthovanadate for 5 minutes cell lysate, Lane 3: PC-12 cell lysate, Lane 4: PC-12 treated with 1mM sodium orthovanadate for 30 minutes cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: Lane 1-2: 20 seconds; Lane 3-4: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
ICC/IF
Immunocytochemistry analysis of Ramos cells serum starved for 16 hours, then treated with 12μg/mL human IgM witch diluted in the serum-free medium for 2 minutes labeling Phospho-LYN (Y397)/LCK (Y394)/HCK (Y411)/BLK (Y389) with Rabbit anti-Phospho-LYN (Y397)/LCK (Y394)/HCK (Y411)/BLK (Y389) antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Phospho-LYN (Y397)/LCK (Y394)/HCK (Y411)/BLK (Y389) antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.| Application Notes | WB:1:2000 ICC:1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
