WB
Western Blot analysis of MOUSE-LIVER cells using Phospho-TGFβ RI (S165) Polyclonal Antibody diluted at 1:1000.IHC
Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1,TGFβ RI (phospho Ser165) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.ICC/IF
Immunofluorescence analysis of HepG2 cells, Antibody diluted at 1:50. The picture on the right is blocked with the synthesized peptide.| Product Name | TGFβ RI (Phospho Ser165) Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The antiserum was produced against synthesized peptide derived from human TGF beta Receptor I around the phosphorylation site of Ser165. AA range:131-180 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:300 ICC:1:50-1:300 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TGFBR1 |
|---|---|
| Gene Synonyms | AAT5 ALK5 ESS1 LDS1 MSSE SKR4 TBRI ALK-5 LDS1A LDS2A TBR-i TGFR-1 ACVRLK4 tbetaR-I |
| Gene Full Name | transforming growth factor beta receptor 1 |
| Gene Summary | The protein encoded by this gene forms a heteromeric complex with type II TGF-beta receptors when bound to TGF-beta, transducing the TGF-beta signal from the cell surface to the cytoplasm. The encoded protein is a serine/threonine protein kinase. Mutations in this gene have been associated with Loeys-Dietz aortic aneurysm syndrome (LDAS). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008] |
| Molecular Weight(MW) | 56kDa |
| Function | Phospho-TGFβ RI (S165) Polyclonal Antibody detects endogenous levels of TGFβ RI protein only when phosphorylated at S165. |
| Cellular Localization | Cell membrane. |
WB
Western Blot analysis of MOUSE-LIVER cells using Phospho-TGFβ RI (S165) Polyclonal Antibody diluted at 1:1000.
IHC
Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1,TGFβ RI (phospho Ser165) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
ICC/IF
Immunofluorescence analysis of HepG2 cells, Antibody diluted at 1:50. The picture on the right is blocked with the synthesized peptide.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:300 ICC:1:50-1:300 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
Data sheet for OM643672