WB
Various lysates were subjected to Tris-acetate gel system followed by western blot with UBR4 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using UBR4 antibody at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).ICC/IF
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using UBR4 antibody at dilution of 1:200 and 488-Conjugated Goat Anti-Rabbit IgG(H+L).IP
IP result of anti-UBR4 (IP:4ug; Detection: 1:2000) with HeLa cells lysate 1.8mg.| Product Name | UBR4 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human UBR4 protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIPWB |
| WB:1:1000-1:4000 IHC:1:200-1:800 ICC:1:50-1:500 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate. |
|
| Species Reactivity | HumanMouse |
| Concentration | 0.26mg/ml |
| Purification | Affinity purified |
| Gene Symbol | UBR4 |
|---|---|
| Gene Synonyms | p600 ZUBR1 RBAF600 |
| Gene Full Name | ubiquitin protein ligase E3 component n-recognin 4 |
| Gene Summary | The protein encoded by this gene is an E3 ubiquitin-protein ligase that interacts with the retinoblastoma-associated protein in the nucleus and with calcium-bound calmodulin in the cytoplasm. The encoded protein appears to be a cytoskeletal component in the cytoplasm and part of the chromatin scaffold in the nucleus. In addition, this protein is a target of the human papillomavirus type 16 E7 oncoprotein. [provided by RefSeq, Aug 2010] |
| Alternative Names | 600 kDa retinoblastoma protein-associated factor E3 ubiquitin-protein ligase UBR4 EC:2.3.2.27 N-recognin-4 p600 |
| Molecular Weight(MW) | 570kDa(Observed Molecular Weight 570kDa,270 kDa) |
| Cellular Localization | Cytoplasm, cytoskeleton,Nucleus. |
WB
Various lysates were subjected to Tris-acetate gel system followed by western blot with UBR4 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using UBR4 antibody at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
ICC/IF
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using UBR4 antibody at dilution of 1:200 and 488-Conjugated Goat Anti-Rabbit IgG(H+L).
IP
IP result of anti-UBR4 (IP:4ug; Detection: 1:2000) with HeLa cells lysate 1.8mg.| Application Notes | WB:1:1000-1:4000 IHC:1:200-1:800 ICC:1:50-1:500 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
