DB
The N6-methyladenosine / m6A Rabbit mAb are tested in Dot Blot against N6 methyladenosine (m6A) and unmodified adenosine. Oligomer 8 - ATAACTGG-m6A-CCGAATGG, Oligomer 9 - ATAACTGGACCGAATGG Oligomer, 10 - AAAAAAAAAAAAAAAA-biotin.ICC/IF
U2OS cells pre-treated with BrdU were subjected UVC irradiation incubated at 37 °C for the indicated time,immunofluorescence analysis was performed by N6 methyladenosine / m6A Rabbit mAb,DAPI, 4′,6-diamidino-2 phenylindole. Global UVC irradiation exceed cytoplasmic leavel,peaking at 2 min after irradiation and diminishing over the following 8 min.| Product Name | N6-methyladenosine / m6A Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Chemical compounds corresponding to N6-methyladenosine / m6A. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | DBICC/IF |
| DB:1:500-1:2000 ICC:1:50-1:200 |
|
| Species Reactivity | All |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | N6-methyladenosine m6A N6-methyladenosine / m6A |
|---|---|
| Function | Discovered in the 1970s, m6A is the most prevalent internal modification in polyadenylated mRNAs and long non-coding RNAs (lncRNAs) in higher eukaryotes. m6A is widely conserved among eukaryotic species that range from yeast, plants, flies to mammals, as well as among viral RNAs with a nuclear phase. The m6A-based modification is associated with a well-defined RNA motif, RRACH (R: A/G, H: A/C/U). As a representative of the epitranscriptome, m6A mRNA modifications participate in many vital activities in the cell, including stem cell self-renewal and differentiation, mRNA transcription, alternative splicing, nuclear export, translation, degradation, and microRNA processing. These processes determine the expression or inactivation of specific genes, which is vital for growth and development.(PMID: 30416848; PMID: 24662220; PMID: 30429466) |
DB
The N6-methyladenosine / m6A Rabbit mAb are tested in Dot Blot against N6 methyladenosine (m6A) and unmodified adenosine. Oligomer 8 - ATAACTGG-m6A-CCGAATGG, Oligomer 9 - ATAACTGGACCGAATGG Oligomer, 10 - AAAAAAAAAAAAAAAA-biotin.
ICC/IF
U2OS cells pre-treated with BrdU were subjected UVC irradiation incubated at 37 °C for the indicated time,immunofluorescence analysis was performed by N6 methyladenosine / m6A Rabbit mAb,DAPI, 4′,6-diamidino-2 phenylindole. Global UVC irradiation exceed cytoplasmic leavel,peaking at 2 min after irradiation and diminishing over the following 8 min.| Application Notes | DB:1:500-1:2000 ICC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
