WB
Western blot analysis of LC3B on different lysates with Rabbit anti-LC3B antibody at 1/1,000 dilution. Lane 1: HCT 116 cell lysate, Lane 2: HCT 116 treated with 50μM Chloroquine for 18 hours cell lysate, Lane 3: U-87 MG cell lysate, Lane 4: C6 cell lysate, Lane 5: Mouse brain tissue lysate, Lane 6: Rat brain tissue lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 26 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue with Rabbit anti-LC3B antibody at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.ICC/IF
Immunocytochemistry analysis of C6 cells treated with or without 50μM Chloroquine for 24 hours labeling LC3B with Rabbit anti-LC3B antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-LC3B antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.| Product Name | LC3B Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within human LC3 B aa 1-20. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:1000-1:2000 IHC:1:1000-1:5000 ICC:1:100-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | MAP1LC3B |
|---|---|
| Gene Synonyms | LC3B ATG8F MAP1LC3B-a MAP1A/1BLC3 |
| Gene Full Name | microtubule associated protein 1 light chain 3 beta |
| Gene Summary | The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component. [provided by RefSeq, Jul 2008] |
| Alternative Names | ATG8F antibody Autophagy-related protein LC3 B antibody Autophagy-related ubiquitin-like modifier LC3 B antibody LC3B antibody LC3II antibody MAP1 light chain 3 like protein 2 antibody MAP1 light chain 3-like protein 2 antibody MAP1A/1BLC3 antibody MAP1A/MAP1B LC3 B antibody MAP1A/MAP1B light chain 3 B antibody ATG8F antibody Autophagy-related protein LC3 B antibody Autophagy-related ubiquitin-like modifier LC3 B antibody LC3B antibody LC3II antibody MAP1 light chain 3 like protein 2 antibody MAP1 light chain 3-like protein 2 antibody MAP1A/1BLC3 antibody MAP1A/MAP1B LC3 B antibody MAP1A/MAP1B light chain 3 B antibody MAP1ALC3 antibody MAP1LC3B a antibody Map1lc3b antibody Microtubule associated protein 1 light chain 3 beta antibody Microtubule-associated protein 1 light chain 3 beta antibody Microtubule-associated proteins 1A/1B light chain 3B antibody MLP3B_HUMAN antibody |
| Molecular Weight(MW) | 14/16 kDa |
| Cellular Localization | Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Membrane, Microtubule, Mitochondrion. |
WB
Western blot analysis of LC3B on different lysates with Rabbit anti-LC3B antibody at 1/1,000 dilution. Lane 1: HCT 116 cell lysate, Lane 2: HCT 116 treated with 50μM Chloroquine for 18 hours cell lysate, Lane 3: U-87 MG cell lysate, Lane 4: C6 cell lysate, Lane 5: Mouse brain tissue lysate, Lane 6: Rat brain tissue lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 26 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue with Rabbit anti-LC3B antibody at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC/IF
Immunocytochemistry analysis of C6 cells treated with or without 50μM Chloroquine for 24 hours labeling LC3B with Rabbit anti-LC3B antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-LC3B antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.| Application Notes | WB:1:1000-1:2000 IHC:1:1000-1:5000 ICC:1:100-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
