WB
Western blot analysis of lysates from wild type (WT) and 293F cells transfected with IL33 using IL33 Rabbit mAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 0.5s.IHC
Immunohistochemistry analysis of paraffin embedded Human tonsil using IL33 Rabbit mAb at dilution of 1:900 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.ICC/IF
Confocal imaging of 293T cells transfected with IL33 using IL33 Rabbit mAb (dilution 1:400) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500)(Red).DAPI was used for nuclear staining (Blue). objective: 100x.| Product Name | IL33 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 109-270 of human IL33 (NP_001300974.1). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:1000 IHC:1:500-1:1000 ICC:1:100-1:500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IL33 |
|---|---|
| Gene Synonyms | DVS27 IL1F11 NF-HEV NFEHEV C9orf26 |
| Gene Full Name | interleukin 33 |
| Gene Summary | The protein encoded by this gene is a cytokine that binds to the IL1RL1/ST2 receptor. The encoded protein is involved in the maturation of Th2 cells and the activation of mast cells, basophils, eosinophils and natural killer cells. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015] |
| Alternative Names | DVS27 IL1F11 NF-HEV NFEHEV C9orf26 IL33 |
| Molecular Weight(MW) | 31kDa(Observed MW 18kDa) |
| Cellular Localization | Chromosome, Cytoplasmic vesicle, Nucleus, Secreted, secretory vesicle |
WB
Western blot analysis of lysates from wild type (WT) and 293F cells transfected with IL33 using IL33 Rabbit mAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 0.5s.
IHC
Immunohistochemistry analysis of paraffin embedded Human tonsil using IL33 Rabbit mAb at dilution of 1:900 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ICC/IF
Confocal imaging of 293T cells transfected with IL33 using IL33 Rabbit mAb (dilution 1:400) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500)(Red).DAPI was used for nuclear staining (Blue). objective: 100x.| Application Notes | WB:1:500-1:1000 IHC:1:500-1:1000 ICC:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
