WB
Positive WB detected in: HepG2 whole cell lysate.
All lanes: Wdr37 antibody at 3.34µg/ml.
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 56 kDa.
Observed band size: 56 kDa.
IHC
IHC image of Wdr37 Rabbit polyclonal antibody diluted at 1:500 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.