WB
Western blot analysis of Serotonin transporter on different lysates with Rabbit anti-Serotonin transporter antibody at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate, Lane 2: Rat brain tissue lysate, Lane 3: Mouse lung tissue lysate, Lysates/proteins at 30 µg/Lane. Exposure time: 28 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.FC
Flow cytometric analysis of HepG2 cells labeling Serotonin transporter. Cells were fixed and permeabilized. Then stained with the primary antibody (1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Product Name | Serotonin transporter Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within Human Serotonin transporter aa 38-87 / 630. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCWB |
| WB:1:1000 FC:1:500-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | SLC6A4 |
|---|---|
| Gene Synonyms | HTT 5HTT OCD1 SERT 5-HTT SERT1 hSERT 5-HTTLPR |
| Gene Full Name | solute carrier family 6 member 4 |
| Gene Summary | This gene encodes an integral membrane protein that transports the neurotransmitter serotonin from synaptic spaces into presynaptic neurons. The encoded protein terminates the action of serotonin and recycles it in a sodium-dependent manner. This protein is a target of psychomotor stimulants, such as amphetamines and cocaine, and is a member of the sodium:neurotransmitter symporter family. A repeat length polymorphism in the promoter of this gene has been shown to affect the rate of serotonin uptake. There have been conflicting results in the literature about the possible effect, if any, that this polymorphism may play in behavior and depression. [provided by RefSeq, May 2019] |
| Alternative Names | 5 HTT antibody 5 HTTLPR antibody 5 hydroxytryptamine (serotonin) transporter antibody 5 hydroxytryptamine transporter antibody 5HT transporter antibody 5HTT antibody hSERT antibody HTT antibody Na+/Cl- dependent serotonin transporter antibody OCD1 antibody 5 HTT antibody 5 HTTLPR antibody 5 hydroxytryptamine (serotonin) transporter antibody 5 hydroxytryptamine transporter antibody 5HT transporter antibody 5HTT antibody hSERT antibody HTT antibody Na+/Cl- dependent serotonin transporter antibody OCD1 antibody SC6A4_HUMAN antibody Serotonin transporter 1 antibody SERT antibody SERT1 antibody Slc6a4 antibody Sodium dependent serotonin transporter antibody Sodium-dependent serotonin transporter antibody Solute carrier family 6 (neurotransmitter transporter) member 4 antibody solute carrier family 6 (neurotransmitter transporter serotonin) member 4 antibody Solute carrier family 6 member 4 antibody |
| Molecular Weight(MW) | 70kDa(Observed band size: 75kDa) |
| Cellular Localization | Cell membrane. Endomembrane system. Endosome membrane. Translocates from intracellular locations to the plasma membrane. Density of transporter molecules on the plasma membrane is itself regulated by serotonin. |
WB
Western blot analysis of Serotonin transporter on different lysates with Rabbit anti-Serotonin transporter antibody at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate, Lane 2: Rat brain tissue lysate, Lane 3: Mouse lung tissue lysate, Lysates/proteins at 30 µg/Lane. Exposure time: 28 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.
FC
Flow cytometric analysis of HepG2 cells labeling Serotonin transporter. Cells were fixed and permeabilized. Then stained with the primary antibody (1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Application Notes | WB:1:1000 FC:1:500-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
