WB
Western blot analysis of various lysates, using BCKDHB Rabbit mAb at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L)at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 0.5s.IHC
Immunohistochemistry analysis of paraffin embedded Human liver using BCKDHB Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffin embedded Mouse kidney using BCKDHB Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.| Product Name | BCKDHB Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 51-392 of human BCKDHB (NP_898871.1). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:1000-1:5000 IHC:1:200-1:800 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BCKDHB |
|---|---|
| Gene Synonyms | E1B OVD1B MSUD1B BCKDE1B BCKDH E1-beta |
| Gene Full Name | branched chain keto acid dehydrogenase E1 subunit beta |
| Gene Summary | This gene encodes the E1 beta subunit of branched-chain keto acid dehydrogenase, which is a multienzyme complex associated with the inner membrane of mitochondria. This enzyme complex functions in the catabolism of branched-chain amino acids. Mutations in this gene have been associated with maple syrup urine disease (MSUD), type 1B, a disease characterized by a maple syrup odor to the urine in addition to mental and physical retardation and feeding problems. Alternative splicing at this locus results in multiple transcript variants. [provided by RefSeq, Jan 2016] |
| Alternative Names | E1B BCKDE1B BCKDH E1-beta BCKDHB |
| Molecular Weight(MW) | 43kDa(Observed MW 37kDa) |
| Cellular Localization | Mitochondrion matrix. |
WB
Western blot analysis of various lysates, using BCKDHB Rabbit mAb at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L)at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 0.5s.
IHC
Immunohistochemistry analysis of paraffin embedded Human liver using BCKDHB Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffin embedded Mouse kidney using BCKDHB Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.| Application Notes | WB:1:1000-1:5000 IHC:1:200-1:800 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
