WB
Western blot analysis of GLB1 / Beta-galactosidase on different lysates with Rabbit anti-GLB1 / Beta-galactosidase antibody at 1/1,000 dilution. Lane 1: SK-Br-3 cell lysate (20 µg/Lane) Lane 2: Saos-2 cell lysate (20 µg/Lane) Lane 3: U-87 MG cell lysate (20 µg/Lane) Lane 4: MCF7 cell lysate (20 µg/Lane) Lane 5: HepG2 cell lysate (20 µg/Lane) Lane 6: K-562 cell lysate (20 µg/Lane) Lane 7: Daudi cell lysate (20 µg/Lane) Lane 8: NIH/3T3 cell lysate (20 µg/Lane) Lane 9: Rat brain tissue lysate (40 µg/Lane) Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded mouse placenta tissue with Rabbit anti-GLB1 / Beta-galactosidase antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.FC
Flow cytometric analysis of SK-Br-3 cells labeling GLB1 / Beta-galactosidase. Cells were fixed and permeabilized. Then stained with the primary antibody (1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Product Name | GLB1 / Beta-galactosidase Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within Human GLB1 aa 628-677 / 677. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCIHCWB |
| WB:1:1000 IHC:1:1000 FC:1:500-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | GLB1 |
|---|---|
| Gene Synonyms | EBP ELNR1 MPS4B |
| Gene Full Name | galactosidase beta 1 |
| Gene Summary | This gene encodes a member of the glycosyl hydrolase 35 family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature lysosomal enzyme. This enzyme catalyzes the hydrolysis of a terminal beta-linked galactose residue from ganglioside substrates and other glycoconjugates. Mutations in this gene may result in GM1-gangliosidosis and Morquio B syndrome. [provided by RefSeq, Nov 2015] |
| Alternative Names | Acid beta galactosidase antibody Acid beta-galactosidase antibody Beta galactosidase 1 antibody Beta galactosidase antibody Beta-galactosidase antibody BGAL_HUMAN antibody EBP antibody EBP included antibody Elastin receptor 1 (67kD) antibody Elastin receptor 1 67kDa antibody Acid beta galactosidase antibody Acid beta-galactosidase antibody Beta galactosidase 1 antibody Beta galactosidase antibody Beta-galactosidase antibody BGAL_HUMAN antibody EBP antibody EBP included antibody Elastin receptor 1 (67kD) antibody Elastin receptor 1 67kDa antibody Elastin receptor 1 antibody Elastin receptor 1 included antibody Elastin-binding protein included antibody ELNR1 antibody Galactosidase beta 1 antibody GLB 1 antibody GLB1 antibody Lactase antibody MPS4B antibody S-GAL included antibody |
| Molecular Weight(MW) | 76kDa(Observed band size: 76/100kDa) |
| Cellular Localization | Lysosome and Cytoplasm. |
WB
Western blot analysis of GLB1 / Beta-galactosidase on different lysates with Rabbit anti-GLB1 / Beta-galactosidase antibody at 1/1,000 dilution. Lane 1: SK-Br-3 cell lysate (20 µg/Lane) Lane 2: Saos-2 cell lysate (20 µg/Lane) Lane 3: U-87 MG cell lysate (20 µg/Lane) Lane 4: MCF7 cell lysate (20 µg/Lane) Lane 5: HepG2 cell lysate (20 µg/Lane) Lane 6: K-562 cell lysate (20 µg/Lane) Lane 7: Daudi cell lysate (20 µg/Lane) Lane 8: NIH/3T3 cell lysate (20 µg/Lane) Lane 9: Rat brain tissue lysate (40 µg/Lane) Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded mouse placenta tissue with Rabbit anti-GLB1 / Beta-galactosidase antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
FC
Flow cytometric analysis of SK-Br-3 cells labeling GLB1 / Beta-galactosidase. Cells were fixed and permeabilized. Then stained with the primary antibody (1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Application Notes | WB:1:1000 IHC:1:1000 FC:1:500-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
