WB
Western Blot analysis of HL-60 and SW-620 cell lysates (35ug) by using anti-CD4 monoclonal antibody.WB
Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Colon, 9: Spleen, 10: Thyroid). Diluation: 1:500.IHC
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-CD4 mouse monoclonal antibody. (heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)ICC/IF
Immunofluorescent staining of Jurkat cells using anti-CD4 mouse monoclonal antibody (green, 1:50). Actin filaments were labeled with Alexa Fluor® 594 Phalloidin (red), and nuclear with DAPI (blue). Scale bar, 4µm.| Product Name | CD4 Mouse Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Full length human recombinant protein of human CD4 (NP_000607) produced in HEK293T cell. |
| Clonality | monoclonal |
|---|---|
| Isotype | Ig2b |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500 IHC:1:50-1:100 ICC/IF:1:50 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A/G |
| Gene Symbol | CD4 |
|---|---|
| Gene Synonyms | T4 IMD79 Leu-3 OKT4D CD4mut |
| Gene Full Name | CD4 molecule |
| Gene Summary | This gene encodes the CD4 membrane glycoprotein of T lymphocytes. The CD4 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class II MHC molecules. The CD4 antigen is also a primary receptor for entry of the human immunodeficiency virus through interactions with the HIV Env gp120 subunit. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, granulocytes, as well as in various regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq, May 2020] |
| Alternative Names | CD4mut IMD79 OKT4D |
| Molecular Weight(MW) | 51kDa |
| Cellular Localization | Cell membrane. |
WB
Western Blot analysis of HL-60 and SW-620 cell lysates (35ug) by using anti-CD4 monoclonal antibody.
WB
Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Colon, 9: Spleen, 10: Thyroid). Diluation: 1:500.
IHC
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-CD4 mouse monoclonal antibody. (heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)
ICC/IF
Immunofluorescent staining of Jurkat cells using anti-CD4 mouse monoclonal antibody (green, 1:50). Actin filaments were labeled with Alexa Fluor® 594 Phalloidin (red), and nuclear with DAPI (blue). Scale bar, 4µm.| Application Notes | WB:1:500 IHC:1:50-1:100 ICC/IF:1:50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C as received. |
| Storage Buffer | PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
