WB
Western blot analysis of various lysates, using ACOX1 Rabbit mAb at 1:120000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.IHC
Immunohistochemistry analysis of paraffin embedded mouse kidney using ACOX1 Rabbit mAb at dilution of 1:1000 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.ICC/IF
Confocal imaging of Hep G2 cells using ACOX1 Rabbit mAb (dilution 1:600)(Red). The cells were counterstained with α-Tubulin Mouse mAb (dilution 1:400) (Green).DAPI was used for nuclear staining (blue). Objective: 60x.| Product Name | ACOX1 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human ACOX1 (NP_004026.2). |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:10000-1:140000 IHC:1:500-1:1000 ICC/IF:1:500-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ACOX1 |
|---|---|
| Gene Synonyms | AOX ACOX SCOX MITCH PALMCOX |
| Gene Full Name | acyl-CoA oxidase 1 |
| Gene Summary | The protein encoded by this gene is the first enzyme of the fatty acid beta-oxidation pathway, which catalyzes the desaturation of acyl-CoAs to 2-trans-enoyl-CoAs. It donates electrons directly to molecular oxygen, thereby producing hydrogen peroxide. Defects in this gene result in pseudoneonatal adrenoleukodystrophy, a disease that is characterized by accumulation of very long chain fatty acids. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008] |
| Molecular Weight(MW) | 50kDa/72kDa |
| Cellular Localization | Peroxisome |
WB
Western blot analysis of various lysates, using ACOX1 Rabbit mAb at 1:120000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
IHC
Immunohistochemistry analysis of paraffin embedded mouse kidney using ACOX1 Rabbit mAb at dilution of 1:1000 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ICC/IF
Confocal imaging of Hep G2 cells using ACOX1 Rabbit mAb (dilution 1:600)(Red). The cells were counterstained with α-Tubulin Mouse mAb (dilution 1:400) (Green).DAPI was used for nuclear staining (blue). Objective: 60x.| Application Notes | WB:1:10000-1:140000 IHC:1:500-1:1000 ICC/IF:1:500-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
