WB
Western blot analysis of various lysates using FXR/NR1H4 Rabbit mAb at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.IHC
Immunohistochemistry analysis of paraffin embedded Rat kidney using FXR/NR1H4 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffin embedded Mouse colon using FXR/NR1H4 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.WB
(E) Representative immunoblot of FXR protein level in HepG2 or Huh-7 cell transfected with miR-382-5p-Ago (100 nM), miR-382-5p-ANT (200 nM) or corresponding control for 36 h with the treatment of GW4064 (2 μM) for 24 h till the harvest, normalized to β-actin protein level.| Product Name | FXR/NR1H4 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description |
|
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human FXR/NR1H4 (NP_001193922.1). |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:1000-1:2000 IHC:1:200-1:800 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NR1H4 |
|---|---|
| Gene Synonyms | BAR FXR HRR1 HRR-1 PFIC5 RIP14 |
| Gene Full Name | nuclear receptor subfamily 1 group H member 4 |
| Gene Summary | This gene encodes a ligand-activated transcription factor that shares structural features in common with nuclear hormone receptor family members. This protein functions as a receptor for bile acids, and when bound to bile acids, binds to DNA and regulates the expression of genes involved in bile acid synthesis and transport. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Feb 2016] |
| Alternative Names | BAR FXR HRR1 HRR-1 PFIC5 RIP14 FXR/NR1H4 |
| Molecular Weight(MW) | 57kDa |
| Cellular Localization | Nucleus. |
WB
Western blot analysis of various lysates using FXR/NR1H4 Rabbit mAb at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
IHC
Immunohistochemistry analysis of paraffin embedded Rat kidney using FXR/NR1H4 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffin embedded Mouse colon using FXR/NR1H4 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
WB
(E) Representative immunoblot of FXR protein level in HepG2 or Huh-7 cell transfected with miR-382-5p-Ago (100 nM), miR-382-5p-ANT (200 nM) or corresponding control for 36 h with the treatment of GW4064 (2 μM) for 24 h till the harvest, normalized to β-actin protein level.| Application Notes | WB:1:1000-1:2000 IHC:1:200-1:800 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
