WB
Western blot analysis of NR1H4 using anti-NR1H4 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NR1H4 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.ICC/IF
ICC/IF analysis of NR1H4 using anti-NR1H4 antibody. NR1H4 was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-NR1H4 Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).FC
Flow Cytometry analysis of A549 cells using anti-NR1H4 antibody. Overlay histogram showing A549 cells stained with Rabbit polyclonal antibody to NR1H4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NR1H4 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Rabbit polyclonal antibody to NR1H4 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human NR1H4, identical to the related mouse and rat sequences. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFWB |
| WB:1:500-1:2000 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NR1H4 |
|---|---|
| Gene Synonyms | BAR FXR HRR1 HRR-1 PFIC5 RIP14 |
| Gene Full Name | nuclear receptor subfamily 1 group H member 4 |
| Gene Summary | This gene encodes a ligand-activated transcription factor that shares structural features in common with nuclear hormone receptor family members. This protein functions as a receptor for bile acids, and when bound to bile acids, binds to DNA and regulates the expression of genes involved in bile acid synthesis and transport. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Feb 2016] |
| Alternative Names | BAR Bile acid receptor Farnesoid X activated receptor Farnesol receptor HRR 1 FXR HRR 1 HRR1 NR1H4 RIP14 RXR interacting protein 14 |
| Molecular Weight(MW) | 56 kDa |
| Cellular Localization | Nucleus. |
WB
Western blot analysis of NR1H4 using anti-NR1H4 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NR1H4 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
ICC/IF
ICC/IF analysis of NR1H4 using anti-NR1H4 antibody. NR1H4 was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-NR1H4 Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).
FC
Flow Cytometry analysis of A549 cells using anti-NR1H4 antibody. Overlay histogram showing A549 cells stained with Rabbit polyclonal antibody to NR1H4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NR1H4 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-1:2000 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
