WB
Various lysates were subjected to SDS PAGE followed by western blot with Beclin 1 antibody at dilution of 1:6000 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using Beclin 1 Antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).ICC/IF
Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using Beclin 1 antibody at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).IF-P
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using Beclin 1 antibody at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IP
IP result of anti-Beclin 1 (IP: 4ug; Detection: 1:1000) with MDA-MB-453s cells lysate 2800 ug.| Product Name | Beclin 1 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human Beclin 1 protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIF-PIHCIPWB |
| WB:1:1000-1:10000 IHC:1:50-1:500 ICC/IF:1:200-1:800 IF-P:1:50-1:500 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BECN1 |
|---|---|
| Gene Synonyms | ATG6 VPS30 beclin1 |
| Gene Full Name | beclin 1 |
| Gene Summary | This gene encodes a protein that regulates autophagy, a catabolic process of degradation induced by starvation. The encoded protein is a component of the phosphatidylinositol-3-kinase (PI3K) complex which mediates vesicle-trafficking processes. This protein is thought to play a role in multiple cellular processes, including tumorigenesis, neurodegeneration and apoptosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015] |
| Alternative Names | BECN1 Beclin-1-C 37 kDa Beclin-1-C 35 kDa Beclin-1 Beclin1 |
| Molecular Weight(MW) | 52-60kDa |
| Cellular Localization | Cytoplasm. |
WB
Various lysates were subjected to SDS PAGE followed by western blot with Beclin 1 antibody at dilution of 1:6000 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using Beclin 1 Antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
ICC/IF
Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using Beclin 1 antibody at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF-P
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using Beclin 1 antibody at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP
IP result of anti-Beclin 1 (IP: 4ug; Detection: 1:1000) with MDA-MB-453s cells lysate 2800 ug.| Application Notes | WB:1:1000-1:10000 IHC:1:50-1:500 ICC/IF:1:200-1:800 IF-P:1:50-1:500 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
