WB
Western blot analysis of Acetylcholinesterase on different lysates with Rabbit anti-Acetylcholinesterase antibody at 1/5,000 dilution. Lane 1: Mouse brain tissue lysate, Lane 2: Mouse liver tissue lysate (negative), Lane 3: Rat brain tissue lysate, Lane 4: Rat liver tissue lysate (negative), Lysates/proteins at 20 µg/Lane. Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded mouse striatum tissue with Rabbit anti-Acetylcholinesterase antibody at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IF-F
Immunofluorescence analysis of frozen mouse striatum tissue with Rabbit anti-Acetylcholinesterase antibody at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).| Product Name | Acetylcholinesterase Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-FIHCWB |
| WB:1:5000 IHC:1:100 IF-F:1:500 |
|
| Species Reactivity | MouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | Ache |
|---|---|
| Gene Synonyms | ACEE, ARACHE, N-ACHE, YT |
| Gene Full Name | acetylcholinesterase |
| Gene Summary | Enables acetylcholinesterase activity; identical protein binding activity; and laminin binding activity. Involved in positive regulation of cold-induced thermogenesis. Acts upstream of or within several processes, including acetylcholine catabolic process; acetylcholine receptor signaling pathway; and regulation of receptor recycling. Located in several cellular components, including basement membrane; cell surface; and neuromuscular junction. Is expressed in several structures, including alimentary system; genitourinary system; musculature; nervous system; and sensory organ. Human ortholog(s) of this gene implicated in Alzheimer's disease; drug dependence (multiple); epilepsy; myasthenia gravis; and subacute delirium. Orthologous to human ACHE (acetylcholinesterase (Yt blood group)). [provided by Alliance of Genome Resources, Feb 2025] |
| Alternative Names | ACEE antibody ACES_HUMAN antibody Acetylcholinesterase antibody AChE antibody Apoptosis related acetylcholinesterase antibody ARACHE antibody N ACHE antibody N-ACHE antibody YT antibody YT blood group antibody |
| Molecular Weight(MW) | 68kDa |
| Cellular Localization | Synapse, Secreted, Cell membrane. |
WB
Western blot analysis of Acetylcholinesterase on different lysates with Rabbit anti-Acetylcholinesterase antibody at 1/5,000 dilution. Lane 1: Mouse brain tissue lysate, Lane 2: Mouse liver tissue lysate (negative), Lane 3: Rat brain tissue lysate, Lane 4: Rat liver tissue lysate (negative), Lysates/proteins at 20 µg/Lane. Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded mouse striatum tissue with Rabbit anti-Acetylcholinesterase antibody at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IF-F
Immunofluorescence analysis of frozen mouse striatum tissue with Rabbit anti-Acetylcholinesterase antibody at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).| Application Notes | WB:1:5000 IHC:1:100 IF-F:1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
