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CD3 epsilon Recombinant Rabbit Monoclonal Antibody
Catalog Number:OM643918
OM643918
Discount Price
OM643918-100ul
2~3 Week
100ul
OM643918-50ul
2~3 Week
50ul
OM643918-20ul
2~3 Week
20ul
2~3 Week
Product Profile
Product Name CD3 epsilon Recombinant Rabbit Monoclonal Antibody
Antibody Type Primary Antibodies
Immunogen Recombinant protein within human EIF2S1 aa 1-315 / 315.
Key Feature
Clonality monoclonal
Isotype IgG
Host Species Rabbit
Tested Applications FCICC/IFIHCWB
WB:1:500-1:2000
IHC:1:1000
ICC/IF:1:50-1:100
FC:1:1000
Species Reactivity Human
Concentration 1mg/ml
Purification Protein A
Target Information
Gene Symbol CD3E
Gene Synonyms T3E
TCRE
IMD18
CD3epsilon
Gene Full Name CD3 epsilon subunit of T-cell receptor complex
Gene Summary The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. [provided by RefSeq, Jul 2008]
Molecular Weight(MW) 23kDa
Cellular Localization Cell membrane.
Application

WB

Western blot analysis of CD3 epsilon on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Human thymus tissue lysate. Lane 2: Jurkat cell lysate.

IHC

Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-CD3 epsilon antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

ICC/IF

Immunocytochemistry analysis of Jurkat cells labeling CD3 epsilon with Rabbit anti-CD3 epsilon antibody at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-CD3 epsilon antibody at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.

FC

Flow cytometric analysis of Jurkat cells labeling CD3 epsilon. Cells were fixed and permeabilized. Then stained with the primary antibody (1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Application Notes WB:1:500-1:2000
IHC:1:1000
ICC/IF:1:50-1:100
FC:1:1000
Additional Information
Form Liquid
Storage Instructions Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage Buffer 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
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