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Rabbit polyclonal antibody to CD3E
Catalog Number:OM644230
OM644230
Discount Price
OM644230-100ul
100ul
OM644230-50ul
50ul
Product Profile
Product Name Rabbit polyclonal antibody to CD3E
Antibody Type Primary Antibodies
Immunogen A synthesized peptide derived from human CD3E(Accession P07766), corresponding to amino acid residues Q172-I207.
Key Feature
Clonality polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications IHCWB
WB:1:500-1:2000
IHC:1:50-1:200
Species Reactivity HumanMouseRat
Concentration 1mg/ml
Purification Affinity purified
Target Information
Gene Symbol CD3E
Gene Synonyms T3E
TCRE
IMD18
CD3epsilon
Gene Full Name CD3 epsilon subunit of T-cell receptor complex
Gene Summary The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. [provided by RefSeq, Jul 2008]
Molecular Weight(MW) 23kDa
Cellular Localization Cell membrane>Single-pass type I membrane protein.
Application

WB

Western blot analysis of extracts from rat muscle, using CD3E Antibody.

IHC

CD3E Antibody at 1/100 staining Human prostate cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.
Application Notes WB:1:500-1:2000
IHC:1:50-1:200
Additional Information
Form Liquid
Storage Instructions Store at -20 °C. Stable for 12 months from date of receipt.
Storage Buffer Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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