WB
Western blot analysis using HPV16 E7 antibody against Human E7 (AA: 1-98) recombinant protein.IHC
Immunohistochemical analysis of paraffin-embedded human placenta tissues (left) and ovarian cancer (right) using E7 mouse mAb with DAB staining.| Product Name | Mouse Monoclonal Antibody to E7 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Purified recombinant fragment of E7 expressed in E. Coli. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | IHCWB |
| WB:1:500-1:2000 IHC:1:200-1:1000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | E7 |
|---|---|
| Gene Synonyms | HpV16gp2 |
| Gene Full Name | transforming protein E7 |
| Alternative Names | E7 HpV16gp2 |
| Molecular Weight(MW) | 21kDa |
| Function | Plays a role in viral genome replication by driving entry of quiescent cells into the cell cycle. Stimulation of progression from G1 to S phase allows the virus to efficiently use the cellular DNA replicating machinery to achieve viral genome replication. E7 protein has both transforming and trans-activating activities. Induces the disassembly of the E2F1 transcription factor from RB1, with subsequent transcriptional activation of E2F1-regulated S-phase genes. Interferes with host histone deacetylation mediated by HDAC1 and HDAC2, leading to transcription activation. Also plays a role in the inhibition of both antiviral and antiproliferative functions of host interferon alpha. Interaction with host TMEM173/STING impairs the ability of TMEM173/STING to sense cytosolic DNA and promote the production of type I interferon (IFN-alpha and IFN-beta). |
| Cellular Localization | Host cytoplasm,Host nucleus. |
WB
Western blot analysis using HPV16 E7 antibody against Human E7 (AA: 1-98) recombinant protein.
IHC
Immunohistochemical analysis of paraffin-embedded human placenta tissues (left) and ovarian cancer (right) using E7 mouse mAb with DAB staining.| Application Notes | WB:1:500-1:2000 IHC:1:200-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide. |
