WB
Western blot analysis of extracts from various samples ,using Histone H2B mouse monoclonal antibody. lane1:K562, lane2:PC12, lane3:mouse brain, lane4:rat brain.IHC
Histone H2B antibody at 1/100 staining Rat heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Mouse antibody was used as the secondary antibody.ICC/IF
Histone H2B antibody staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab and rabbit anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-mouse IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-rabbit IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue).| Product Name | Mouse monoclonal antibody to Histone H2B |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Purified recombinant fragment of human Histone H2B expressed in E. Coli. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:100-1:1000 ICC/IF:1:100-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | H2BC11 |
|---|---|
| Gene Synonyms | H2BJ H2B/r H2BFR HIST1H2BJ |
| Gene Full Name | H2B clustered histone 11 |
| Gene Summary | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2B family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the histone microcluster on chromosome 6p21.33. [provided by RefSeq, Aug 2015] |
| Alternative Names | dJ221C16.8 H2B histone family member A H2B/a H2B/g H2B/h H2B/k H2B/l H2B1C_HUMAN H2BFA HIST1H2BI Histone 1 H2bg Histone cluster 1 H2B family member g Histone cluster 1 H2bg Histone H2B type 1-C/E/F/G/I Histone H2B.1 A Histone H2B.a Histone H2B.g Histone H2B.h Histone H2B.k Histone H2B.l H2B histone family member R H2B/r H2B1J_HUMAN H2BFR HIST1H2BJ Histone 1 H2bj Histone cluster 1 H2bj Histone H2B type 1-J Histone H2B.1 Histone H2B.r GL105 H2B H2B histone family member Q H2B-GL105 H2B.1 H2B/q H2B2E_HUMAN H2BFQ H2BGL105 H2BQ HIST2H2BE Histone 2 H2be histone cluster 2 H2be histone H2B GL105 histone H2B type 2 E Histone H2B type 2-E histone H2B type 2E Histone H2B-GL105 Histone H2B.q histone H2BGL105 H2B K H2B type 12 H2B/b H2B/c H2B/d H2B/e H2B/f H2B/j H2B/n H2B/q H2B/r H2B/s HIRA-interacting protein 1 HIRA-interacting protein 2 Histone H2B type 1-B Histone H2B type 1-C/E/F/G/I Histone H2B type 1-D Histone H2B type 1-H Histone H2B type 1-J Histone H2B type 1-K Histone H2B type 1-L Histone H2B type 1-M Histone H2B type 1-N Histone H2B type 1-O Histone H2B type 2-E Histone H2B type 2-F Histone H2B type 3-B Histone H2B type F-S Histone H2B-GL105 Histone H2B.1 A Histone H2B.1 Histone H2B.1 B Histone H2B.2 Histone H2B.a Histone H2B.b Histone H2B.c Histone H2B.d Histone H2B.e Histone H2B.f Histone H2B.g Histone H2B.h Histone H2B.j Histone H2B.k Histone H2B.l Histone H2B.n Histone H2B.r Histone H2B.s |
| Molecular Weight(MW) | 14kDa |
| Cellular Localization | Nucleus, Chromosome. |
WB
Western blot analysis of extracts from various samples ,using Histone H2B mouse monoclonal antibody. lane1:K562, lane2:PC12, lane3:mouse brain, lane4:rat brain.
IHC
Histone H2B antibody at 1/100 staining Rat heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Mouse antibody was used as the secondary antibody.
ICC/IF
Histone H2B antibody staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab and rabbit anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-mouse IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-rabbit IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue).| Application Notes | WB:1:500-1:2000 IHC:1:100-1:1000 ICC/IF:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C. Stable for 12 months from date of receipt. |
| Storage Buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
