WB
Western blot analysis of Phospho-GSK3 alpha (S21) on different lysates with Rabbit anti-Phospho-GSK3 alpha (S21) antibody at 1/2,000 dilution. Lane 1: A549 cell lysate, Lane 2: A549 treated with 100nM Calyculin A for 30 minutes cell lysate, Lane 3: SH-SY5Y cell lysate, Lane 4: SH-SY5Y treated with 200nM Calyculin A and 1μM Okadaic Acid for 1 hour cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.WB
Western blot analysis of Phospho-GSK3 alpha (S21) on different lysates with Rabbit anti-Phospho-GSK3 alpha (S21) antibody at 1/2,000 dilution. Lane 1: NIH/3T3 starved overnight cell lysate, Lane 2: NIH/3T3 starved overnight then treated with 100ng/mL PDGF for 5 minutes cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-Phospho-GSK3 alpha (S21) antibody at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-Phospho-GSK3 alpha (S21) antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.DB
Dot blot analysis of Phospho-GSK3 alpha (S21) on different peptides with Rabbit anti-Phospho-GSK3 alpha (S21) antibody at 1/1,000 dilution. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution for 1 hour at room temperature. Lane 1: Phospho-GSK3 alpha (S21) peptide (positive), Lane 2: Unmodified GSK3 alpha peptide (negative), Lane 3: Phospho-GSK3 beta (S9) peptide (negative), Lane 4: Unmodified GSK3 beta peptide (negative), Proteins loading: 100ng, 25ng, 5ng; Blocking and dilution buffer: 5% NFDM/TBST; Exposure time: 10 seconds.| Product Name | Phospho-GSK3 alpha (S21) Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic phospho-peptide corresponding to residues surrounding Ser21 of Human GSK3 alpha. |
| Modification | p-S21 |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | DBIHCWB |
| WB:1:2000 IHC:1:200-1:500 DB:1:1000 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | GSK3A |
|---|---|
| Gene Full Name | glycogen synthase kinase 3 alpha |
| Gene Summary | This gene encodes a multifunctional Ser/Thr protein kinase that is implicated in the control of several regulatory proteins including glycogen synthase, and transcription factors, such as JUN. It also plays a role in the WNT and PI3K signaling pathways, as well as regulates the production of beta-amyloid peptides associated with Alzheimer's disease. [provided by RefSeq, Oct 2011] |
| Alternative Names | DKFZp686D0638 antibody Glycogen synthase kinase 3 alpha antibody Glycogen synthase kinase-3 alpha antibody GSK 3 alpha antibody GSK 3A antibody GSK-3 alpha antibody Gsk3a antibody GSK3A_HUMAN antibody GSK3alpha antibody Serine/threonine protein kinase GSK3A antibody |
| Molecular Weight(MW) | 51kDa |
| Function | Glycogen synthase kinase-3 alpha is an enzyme that in humans is encoded by the GSK3A gene. Glycogen synthase kinase 3-alpha EC 2.7.1.37 is a multifunctional protein serine kinase, homologous to Drosophila 'shaggy' (zeste-white3) and implicated in the control of several regulatory proteins including glycogen synthase and various transcription factors (e.g., JUN). It also plays a role in the WNT and phosphoinositide 3-kinase (especially PIK3CG) signaling pathways. |
| Cellular Localization | Apical dendrite, axon, cytoplasm, cytosol, mitochondrion, neuronal cell body, nucleus, postsynapse, proximal dendrite. |
WB
Western blot analysis of Phospho-GSK3 alpha (S21) on different lysates with Rabbit anti-Phospho-GSK3 alpha (S21) antibody at 1/2,000 dilution. Lane 1: A549 cell lysate, Lane 2: A549 treated with 100nM Calyculin A for 30 minutes cell lysate, Lane 3: SH-SY5Y cell lysate, Lane 4: SH-SY5Y treated with 200nM Calyculin A and 1μM Okadaic Acid for 1 hour cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
WB
Western blot analysis of Phospho-GSK3 alpha (S21) on different lysates with Rabbit anti-Phospho-GSK3 alpha (S21) antibody at 1/2,000 dilution. Lane 1: NIH/3T3 starved overnight cell lysate, Lane 2: NIH/3T3 starved overnight then treated with 100ng/mL PDGF for 5 minutes cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-Phospho-GSK3 alpha (S21) antibody at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-Phospho-GSK3 alpha (S21) antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
DB
Dot blot analysis of Phospho-GSK3 alpha (S21) on different peptides with Rabbit anti-Phospho-GSK3 alpha (S21) antibody at 1/1,000 dilution. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution for 1 hour at room temperature. Lane 1: Phospho-GSK3 alpha (S21) peptide (positive), Lane 2: Unmodified GSK3 alpha peptide (negative), Lane 3: Phospho-GSK3 beta (S9) peptide (negative), Lane 4: Unmodified GSK3 beta peptide (negative), Proteins loading: 100ng, 25ng, 5ng; Blocking and dilution buffer: 5% NFDM/TBST; Exposure time: 10 seconds.| Application Notes | WB:1:2000 IHC:1:200-1:500 DB:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
