WB
Various whole cell lysates were separated by 4-8% SDS-PAGE, and the membrane was blotted with anti-Collagen III antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Hela ,Lane 2: Rat womb ,Lane 3: A431.WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4°C, over night with a 1:5000 dilution . The Dylight 800-conjugated Goat anti-Rabbit antibody was used to detect the antibody. Lane1: HL60 - Human promyelocytic leukemia cell Lane2: HUVEC - Human umbilical vein endothelial cell Lane3: Human CAF - Human cancer-associated fibroblast Lane4: HCT116 - Human colorectal carcinoma Lane5: A549 - Human lung carcinoma Lane6: Jurkat - Human T lymphocyte leukemia Lane7: HT22 - Mouse hippocampal neuronal Lane8: Thp-1 - Human monocytic leukemia Lane9: HK-2 - Human proximal tubular epithelial Lane10: PC-12 - Rat adrenal pheochromocytoma Lane11: 3T3-L1 - Mouse embryonic fibroblast Lane12: PC-3 - Human prostate adenocarcinoma Lane13: U-14 - Mouse cervical carcinoma Lane14: 293F - HEK293 derivative, adapted for suspension culture Predicted band size: 138kDa Observed band size: 150kDa.IHC
Rat skin was stained with Anti-Collagen III rabbit antibody.IHC
Human skin was stained with Anti-Collagen III rabbit antibodyICC/IF
Immunofluorescence analysis of HEK293. Picture A: Collagen III antibody (red). Picture B: DAPI (blue). Picture C: Merge of A+B.IF-P
Rat skin was stained with Anti-Collagen III rabbit antibody.| Product Name | Collagen III Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIF-PIHCWB |
| WB:1:1000-1:5000 IHC:1:20-1:100 ICC/IF:1:200-1:1000 IF-P:1:200-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | COL3A1 |
|---|---|
| Gene Synonyms | EDS4A EDSVASC PMGEDSV |
| Gene Full Name | collagen type III alpha 1 chain |
| Gene Summary | This gene encodes the pro-alpha1 chains of type III collagen, a fibrillar collagen that is found in extensible connective tissues such as skin, lung, uterus, intestine and the vascular system, frequently in association with type I collagen. Mutations in this gene are associated with Ehlers-Danlos syndrome type IV, and with aortic and arterial aneurysms. [provided by R. Dalgleish, Feb 2008] |
| Molecular Weight(MW) | 138kDa(Observed band size: 150kDa) |
| Cellular Localization | Cytoplasm. |
WB
Various whole cell lysates were separated by 4-8% SDS-PAGE, and the membrane was blotted with anti-Collagen III antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Hela ,Lane 2: Rat womb ,Lane 3: A431.
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4°C, over night with a 1:5000 dilution . The Dylight 800-conjugated Goat anti-Rabbit antibody was used to detect the antibody. Lane1: HL60 - Human promyelocytic leukemia cell Lane2: HUVEC - Human umbilical vein endothelial cell Lane3: Human CAF - Human cancer-associated fibroblast Lane4: HCT116 - Human colorectal carcinoma Lane5: A549 - Human lung carcinoma Lane6: Jurkat - Human T lymphocyte leukemia Lane7: HT22 - Mouse hippocampal neuronal Lane8: Thp-1 - Human monocytic leukemia Lane9: HK-2 - Human proximal tubular epithelial Lane10: PC-12 - Rat adrenal pheochromocytoma Lane11: 3T3-L1 - Mouse embryonic fibroblast Lane12: PC-3 - Human prostate adenocarcinoma Lane13: U-14 - Mouse cervical carcinoma Lane14: 293F - HEK293 derivative, adapted for suspension culture Predicted band size: 138kDa Observed band size: 150kDa.
IHC
Rat skin was stained with Anti-Collagen III rabbit antibody.
IHC
Human skin was stained with Anti-Collagen III rabbit antibody
ICC/IF
Immunofluorescence analysis of HEK293. Picture A: Collagen III antibody (red). Picture B: DAPI (blue). Picture C: Merge of A+B.
IF-P
Rat skin was stained with Anti-Collagen III rabbit antibody.| Application Notes | WB:1:1000-1:5000 IHC:1:20-1:100 ICC/IF:1:200-1:1000 IF-P:1:200-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
