WB
Various lysates were subjected to SDS PAGE followed by western blot with PLCH1 antibody at dilution of 1:500 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded human brain using PLCH1 antibody at dilution of 1:50 (under 40x lens).ICC/IF
Immunofluorescent analysis of Hela cells, using PLCH1 antibody at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).| Product Name | PLCH1 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human PLCH1 protein |
| Clonality | polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:1000 IHC:1:20-1:200 ICC/IF:1:10-1:100 |
|
| Species Reactivity | Human |
| Concentration | 0.4mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PLCH1 |
|---|---|
| Gene Synonyms | HPE14 PLCL3 PLC-L3 PLC eta 1 |
| Gene Full Name | phospholipase C eta 1 |
| Gene Summary | PLCH1 is a member of the PLC-eta family of the phosphoinositide-specific phospholipase C (PLC) superfamily of enzymes that cleave phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) to generate second messengers inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG) (Hwang et al., 2005 [PubMed 15702972]).[supplied by OMIM, Jun 2009] |
| Molecular Weight(MW) | 185kDa |
| Cellular Localization | Cytoplasm,Membrane. |
WB
Various lysates were subjected to SDS PAGE followed by western blot with PLCH1 antibody at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded human brain using PLCH1 antibody at dilution of 1:50 (under 40x lens).
ICC/IF
Immunofluorescent analysis of Hela cells, using PLCH1 antibody at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).| Application Notes | WB:1:500-1:1000 IHC:1:20-1:200 ICC/IF:1:10-1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Data sheet for OM643991