WB
Adult rat brain membrane (RBM) and transfected cell immunoblot: extracts of RBM (left lane) and COS-1 cells transiently transfected with KCNQ4 (middle lane) or Kv2.1 (right lane) plasmids and probed with pure N43/6 (left panel) or K89/41 TC supe (right panel).ICC/IF
Transfected cell immunofluorescence: COS-1 cell expressing KCNQ4 in a field of untransfected cells. Red = N43/6, Blue = Hoechst nuclear stain.| Product Name | KCNQ4 Mouse Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Fusion protein amino acids 2-77 of human KCNQ4 (Kv7.4, KvLQT4, accession number P56696). Mouse: 94% identity (71/75 amino acids identical). Rat: 94% identity (71/75 amino acids identical). |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ICC/IFWB |
| Species Reactivity | HumanMonkeyRat |
| Purification | Affinity purified |
| Gene Symbol | KCNQ4 |
|---|---|
| Gene Synonyms | DFNA2 KV7.4 DFNA2A |
| Gene Full Name | potassium voltage-gated channel subfamily Q member 4 |
| Gene Summary | The protein encoded by this gene forms a potassium channel that is thought to play a critical role in the regulation of neuronal excitability, particularly in sensory cells of the cochlea. The current generated by this channel is inhibited by M1 muscarinic acetylcholine receptors and activated by retigabine, a novel anti-convulsant drug. The encoded protein can form a homomultimeric potassium channel or possibly a heteromultimeric channel in association with the protein encoded by the KCNQ3 gene. Defects in this gene are a cause of nonsyndromic sensorineural deafness type 2 (DFNA2), an autosomal dominant form of progressive hearing loss. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Molecular Weight(MW) | 77kDa |
| Cellular Localization | Basal cell membrane. |
WB
Adult rat brain membrane (RBM) and transfected cell immunoblot: extracts of RBM (left lane) and COS-1 cells transiently transfected with KCNQ4 (middle lane) or Kv2.1 (right lane) plasmids and probed with pure N43/6 (left panel) or K89/41 TC supe (right panel).
ICC/IF
Transfected cell immunofluorescence: COS-1 cell expressing KCNQ4 in a field of untransfected cells. Red = N43/6, Blue = Hoechst nuclear stain.| Form | Liquid |
|---|---|
| Storage Instructions | Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles. |
| Storage Buffer | PBS with 0.02% Proclin 300. |
Data sheet for OM644001