FC
Flow cytometry: 1X10^6 293T cells (negative control,left) and MOLT-4 cells (right) were surface-stained with PE Rabbit anti-Human PD-1/CD279 mAb (5 μl/Test,orange line) or PE Rabbit IgG isotype control (5 μl/Test,blue line). Non-fluorescently stained cells were used as blank control (red line).FC
Flow cytometry: 1X10^6 MOLT-4 cells were surface-stained with PE Rabbit IgG isotype control (5 μl/Test,left) or PE Rabbit anti-Human PD-1/CD279 mAb (5 μl/Test,right).FC
Flow cytometry: 1X10^6 Human PBMC (negative control,left) and Human PBMC (treated with PHA,right) were surface-stained with PE Rabbit anti-Human PD-1/CD279 mAb (5 μl/Test,orange line) or PE Rabbit IgG isotype control (5 μl/Test,blue line). Non-fluorescently stained cells were used as blank control (red line).FC
Flow cytometry: 1X10^6 Human PBMC (treated with PHA,right) were surface-stained with PE Rabbit IgG isotype control (5 μl/Test,left) or PE Rabbit anti-Human PD-1/CD279 mAb (5 μl/Test,right).| Product Name | PE Rabbit anti-Human PD-1/CD279 mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 25-168 of human PD-1/CD279 (NP_005009.2). |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FC |
| FC:5 μl per 10^6 cells in 100 μl volume |
|
| Species Reactivity | Human |
| Purification | Affinity purified |
| Gene Symbol | PDCD1 |
|---|---|
| Gene Synonyms | PD1 PD-1 CD279 SLEB2 hPD-1 hPD-l hSLE1 AIMTBS |
| Gene Full Name | programmed cell death 1 |
| Gene Summary | Programmed cell death protein 1 (PDCD1) is an immune-inhibitory receptor expressed in activated T cells; it is involved in the regulation of T-cell functions, including those of effector CD8+ T cells. In addition, this protein can also promote the differentiation of CD4+ T cells into T regulatory cells. PDCD1 is expressed in many types of tumors including melanomas, and has demonstrated to play a role in anti-tumor immunity. Moreover, this protein has been shown to be involved in safeguarding against autoimmunity, however, it can also contribute to the inhibition of effective anti-tumor and anti-microbial immunity. [provided by RefSeq, Aug 2020] |
| Alternative Names | PD1 PD-1 CD279 SLEB2 hPD-1 hPD-l hSLE1 |
| Molecular Weight(MW) | 31kDa |
| Cellular Localization | Membrane, external side of plasma membrane, plasma membrane, Single-pass type I membrane protein. |
FC
Flow cytometry: 1X10^6 293T cells (negative control,left) and MOLT-4 cells (right) were surface-stained with PE Rabbit anti-Human PD-1/CD279 mAb (5 μl/Test,orange line) or PE Rabbit IgG isotype control (5 μl/Test,blue line). Non-fluorescently stained cells were used as blank control (red line).
FC
Flow cytometry: 1X10^6 MOLT-4 cells were surface-stained with PE Rabbit IgG isotype control (5 μl/Test,left) or PE Rabbit anti-Human PD-1/CD279 mAb (5 μl/Test,right).
FC
Flow cytometry: 1X10^6 Human PBMC (negative control,left) and Human PBMC (treated with PHA,right) were surface-stained with PE Rabbit anti-Human PD-1/CD279 mAb (5 μl/Test,orange line) or PE Rabbit IgG isotype control (5 μl/Test,blue line). Non-fluorescently stained cells were used as blank control (red line).
FC
Flow cytometry: 1X10^6 Human PBMC (treated with PHA,right) were surface-stained with PE Rabbit IgG isotype control (5 μl/Test,left) or PE Rabbit anti-Human PD-1/CD279 mAb (5 μl/Test,right).| Application Notes | FC:5 μl per 10^6 cells in 100 μl volume |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
