WB
Western blot analysis using HDAC1 mouse mAb against Hela (1), Raw264.7 (2), K562 (3), and Jurkat (4) cell lysate.IHC
Immunohistochemical analysis of paraffin-embedded human colon tissues using HDAC1 mouse mAb with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded human colon cancer tissues using HDAC1 mouse mAb with DAB staining.ICC/IF
Immunofluorescence analysis of Hela cells using HDAC1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)FC
Flow cytometric analysis of Hela cells using HDAC1 mouse mAb (green) and negative control (red).| Product Name | Mouse Monoclonal Antibody to HDAC1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Purified recombinant fragment of human HDAC1 (AA: 321-482) expressed in E. Coli. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:200-1:1000 ICC/IF:1:200-1:1000 FC:1:200-1:400 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HDAC1 |
|---|---|
| Gene Synonyms | HD1 RPD3 KDAC1 GON-10 RPD3L1 |
| Gene Full Name | histone deacetylase 1 |
| Gene Summary | Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis. [provided by RefSeq, Jul 2008] |
| Molecular Weight(MW) | 55kDa |
| Cellular Localization | Nucleus. |
WB
Western blot analysis using HDAC1 mouse mAb against Hela (1), Raw264.7 (2), K562 (3), and Jurkat (4) cell lysate.
IHC
Immunohistochemical analysis of paraffin-embedded human colon tissues using HDAC1 mouse mAb with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded human colon cancer tissues using HDAC1 mouse mAb with DAB staining.
ICC/IF
Immunofluorescence analysis of Hela cells using HDAC1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
FC
Flow cytometric analysis of Hela cells using HDAC1 mouse mAb (green) and negative control (red).| Application Notes | WB:1:500-1:2000 IHC:1:200-1:1000 ICC/IF:1:200-1:1000 FC:1:200-1:400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide. |
Data sheet for OM644031