WB
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PDIA4 monoclonal antibody.IHC
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PDIA4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min)IHC
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-PDIA4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min)| Product Name | ERp72 (PDIA4) Mouse Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Full length human recombinant protein of human PDIA4(NP_004902) produced in HEK293T cell. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | IHCWB |
| WB:1:500-1:2000 IHC:1:150 |
|
| Species Reactivity | DogHumanMonkeyMouseRat |
| Concentration | 0.74 mg/ml |
| Purification | Protein A/G |
| Gene Symbol | PDIA4 |
|---|---|
| Gene Synonyms | ERP70 ERP72 ERp-72 |
| Gene Full Name | protein disulfide isomerase family A member 4 |
| Gene Summary | This gene encodes a member of the disulfide isomerase (PDI) family of endoplasmic reticulum (ER) proteins that catalyze protein folding and thiol-disulfide interchange reactions. The encoded protein has an N-terminal ER-signal sequence, three catalytically active thioredoxin (TRX) domains, two TRX-like domains and a C-terminal ER-retention sequence. This protein, when bound to cyclophilin B, enhances the rate of immunoglobulin G intermolecular disulfide bonding and antibody assembly. [provided by RefSeq, Dec 2016] |
| Molecular Weight(MW) | 72.8kDa |
| Cellular Localization | Endoplasmic reticulum lumen, Melanosome . |
WB
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PDIA4 monoclonal antibody.
IHC
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PDIA4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min)
IHC
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-PDIA4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min)| Application Notes | WB:1:500-1:2000 IHC:1:150 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C as received. Stable for 12 months from date of receipt. |
| Storage Buffer | PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
Data sheet for OM644043