WB
Sample loading: Antigen protein with gradient dilution. Electrophoresis: 12% SDS-PAGE. Membrane transfer: NC (nitrocellulose) membrane, with a voltage of 10 V overnight. Blocking: 5% skimmed milk powder, incubate at room temperature for 1 hour. Primary antibody: Dilute at a ratio of 1:5000, incubate at room temperature for 1 hour.| Product Name | Anti-AHA2/ ATPase 2, plasma membrane-type |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant part protein of Arabidopsis thaliana AT4G30190，P19456 |
| Clonality | polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000-1:5000 |
|
| Species Reactivity | Arabidopsis |
| Purification | Affinity purified |
| Gene Symbol | rpoB |
|---|---|
| Gene Full Name | DNA-directed RNA polymerase subunit beta |
| Target Description | Belongs to the P-type ATPase superfamily of cation-transporting ATPases, pumps protons out of the cell, generating a proton gradient that drives the active transport of nutrients by proton symport. |
| Molecular Weight(MW) | 104.4 kDa for Arabidopsis |
WB
Sample loading: Antigen protein with gradient dilution. Electrophoresis: 12% SDS-PAGE. Membrane transfer: NC (nitrocellulose) membrane, with a voltage of 10 V overnight. Blocking: 5% skimmed milk powder, incubate at room temperature for 1 hour. Primary antibody: Dilute at a ratio of 1:5000, incubate at room temperature for 1 hour.| Application Notes | WB:1:1000-1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 50% glycerol，-20 °C |
Data sheet for OM644062