WB
Western blot analysis of lysates from HeLa cells, using HSF1 Rabbit mAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L)at 1:10000 dilution. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.IHC
Immunohistochemistry analysis of paraffin embedded Mouse testis using HSF1 Rabbit mAb at dilution of 1:300 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.IP
Immunoprecipitation analysis of 300μg extracts of HeLa cells using 3μg HSF1 Rabbit mAb. Western blot was performed from the immunoprecipitate using HSF1 Rabbit mAb at a dilition of 1:2000.| Product Name | HSF1 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 429-530 of human HSF1( NP_005517.1). |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCIPWB |
| WB:1:1000-1:4000 IHC:1:200-1:2000 IP:0.5μg-4μg antibody for 200μg-400μg extracts of whole cells |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HSF1 |
|---|---|
| Gene Synonyms | HSTF1 |
| Gene Full Name | heat shock transcription factor 1 |
| Gene Summary | The product of this gene is a transcription factor that is rapidly induced after temperature stress and binds heat shock promoter elements (HSE). This protein plays a role in the regulation of lifespan. Expression of this gene is repressed by phosphorylation, which promotes binding by heat shock protein 90. [provided by RefSeq, Jul 2017] |
| Molecular Weight(MW) | 57kDa( Observed MW 75kDa) |
| Cellular Localization | Cytoplasm, Nucleus. |
WB
Western blot analysis of lysates from HeLa cells, using HSF1 Rabbit mAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L)at 1:10000 dilution. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
IHC
Immunohistochemistry analysis of paraffin embedded Mouse testis using HSF1 Rabbit mAb at dilution of 1:300 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
IP
Immunoprecipitation analysis of 300μg extracts of HeLa cells using 3μg HSF1 Rabbit mAb. Western blot was performed from the immunoprecipitate using HSF1 Rabbit mAb at a dilition of 1:2000.| Application Notes | WB:1:1000-1:4000 IHC:1:200-1:2000 IP:0.5μg-4μg antibody for 200μg-400μg extracts of whole cells |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
Data sheet for OM644089