WB
Western blot analysis of various lysates using Versican Rabbit mAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L)at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.ICC/IF
Immunofluorescence analysis of NIH-3T3 cells using Versican Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.IHC
Immunohistochemistry analysis of paraffin embedded Human esophageal cancer using Versican Rabbit mAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.| Product Name | IL8 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 3200-3396 of human Versican (P13611). |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:1000-1:2000 ICC/IF:1:100-1:200 IHC:1:100-1:200 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | VCAN |
|---|---|
| Gene Synonyms | WGN ERVR GHAP PG-M WGN1 CSPG2 |
| Gene Full Name | versican |
| Gene Summary | This gene is a member of the aggrecan/versican proteoglycan family. The protein encoded is a large chondroitin sulfate proteoglycan and is a major component of the extracellular matrix. This protein is involved in cell adhesion, proliferation, proliferation, migration and angiogenesis and plays a central role in tissue morphogenesis and maintenance. Mutations in this gene are the cause of Wagner syndrome type 1. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2009] |
| Alternative Names | WGN ERVR GHAP PG-M WGN1 CSPG2 Versican |
| Molecular Weight(MW) | 373kDa(Observed MW 265kDa) |
| Cellular Localization | endoplasmic reticulum lumen, extracellular region, extracellular space, Golgi lumen, interphotoreceptor matrix, lysosomal lumen, photoreceptor outer segment. |
WB
Western blot analysis of various lysates using Versican Rabbit mAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L)at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
ICC/IF
Immunofluorescence analysis of NIH-3T3 cells using Versican Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
IHC
Immunohistochemistry analysis of paraffin embedded Human esophageal cancer using Versican Rabbit mAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.| Application Notes | WB:1:1000-1:2000 ICC/IF:1:100-1:200 IHC:1:100-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
