WB
Staurosporine treated and untreated A2780 cells were subjected to SDS PAGE followed by western blot with Cleaved PARP1 antibody at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with HRP-conjugated Lamin B1 antibody as a loading control.IHC
Immunohistochemical analysis of paraffin-embedded Jurkat (left) and Staurosporine treated Jurkat (right) cells slide using Cleaved PARP1 antibody at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).ICC/IF
Immunofluorescent analysis of (4% PFA) fixed untreated and 1 μM Staurosporine (3 hours) treated HSC-T6 cells using Cleaved PARP1 antibody at dilution of 1:1000 and 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L).WB
a Western blot analysis of Cleaved-Caspase3 and Cleaved-PARP protein expression, and both of them were enhanced following TNF-α treatment. GAPDH was used as a loading control.WB
a Western blot analysis of Cleaved-Caspase3 and Cleaved-PARP protein expression indicated that apoptosis was significantly inhibited by knocking down TIPE2. GAPDH was used as a loading control.| Product Name | Cleaved PARP1 Monoclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Peptide |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCWB |
| WB:1:5000-1:20000 IHC:1:1000-1:4000 ICC/IF:1:1:500-1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | PARP1 |
|---|---|
| Gene Synonyms | PARP PARS PPOL ADPRT ARTD1 ADPRT1 PARP-1 ADPRT 1 pADPRT-1 Poly-PARP |
| Gene Full Name | poly(ADP-ribose) polymerase 1 |
| Gene Summary | This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq, Jul 2008] |
| Alternative Names | PARP1 ARTD1 ADPRT1 ADPRT 1 ADPRT |
| Molecular Weight(MW) | 89kDa |
| Cellular Localization | Chromosome,Cytoplasm,Nucleus. |
WB
Staurosporine treated and untreated A2780 cells were subjected to SDS PAGE followed by western blot with Cleaved PARP1 antibody at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with HRP-conjugated Lamin B1 antibody as a loading control.
IHC
Immunohistochemical analysis of paraffin-embedded Jurkat (left) and Staurosporine treated Jurkat (right) cells slide using Cleaved PARP1 antibody at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
ICC/IF
Immunofluorescent analysis of (4% PFA) fixed untreated and 1 μM Staurosporine (3 hours) treated HSC-T6 cells using Cleaved PARP1 antibody at dilution of 1:1000 and 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L).
WB
a Western blot analysis of Cleaved-Caspase3 and Cleaved-PARP protein expression, and both of them were enhanced following TNF-α treatment. GAPDH was used as a loading control.
WB
a Western blot analysis of Cleaved-Caspase3 and Cleaved-PARP protein expression indicated that apoptosis was significantly inhibited by knocking down TIPE2. GAPDH was used as a loading control.| Application Notes | WB:1:5000-1:20000 IHC:1:1000-1:4000 ICC/IF:1:1:500-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
