WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CAMK2B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CAMK2B (1:2000).IHC
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-CAMK2B mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min) (1:150)| Product Name | Mouse monoclonal antibody to CaMKII Beta/CAMK2B (PhosphoThr287) |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 311-385 of human CAMK2B(NP_001211) produced in E.coli. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | IHCWB |
| WB:1:2000 IHC:1:150 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Protein A/G |
| Gene Symbol | CAMK2B |
|---|---|
| Gene Synonyms | CAM2 CAMK2 CAMKB MRD54 CaMKIIbeta |
| Gene Full Name | calcium/calmodulin dependent protein kinase II beta |
| Gene Summary | The product of this gene belongs to the serine/threonine protein kinase family and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. In mammalian cells, the enzyme is composed of four different chains: alpha, beta, gamma, and delta. The product of this gene is a beta chain. It is possible that distinct isoforms of this chain have different cellular localizations and interact differently with calmodulin. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014] |
| Molecular Weight(MW) | 73kDa(Observed MW 60kDa) |
| Cellular Localization | Cytoplasm,Cytoskeleton,Membrane,Sarcoplasmic reticulum,Synapse |
WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CAMK2B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CAMK2B (1:2000).
IHC
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-CAMK2B mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min) (1:150)| Application Notes | WB:1:2000 IHC:1:150 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
Data sheet for OM644101