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K48-linkage specific ubiquitin Recombinant Rabbit mAb
Catalog Number:OM644109
OM644109
Discount Price
OM644109-100ul
2~3 Week
100ul
OM644109-50ul
50ul
2~3 Week
Product Profile
Product Name K48-linkage specific ubiquitin Recombinant Rabbit mAb
Antibody Type Primary Antibodies
Immunogen KLH conjugated synthetic peptide derived from human AIF.
Key Feature
Clonality monoclonal
Isotype IgG
Host Species Rabbit
Tested Applications IF-PIHCWB
WB:1:500-1:2000
IHC:1:50-1:200
IF-P:1:50-1:200
Species Reactivity HumanMouseRat
Concentration 1mg/ml
Purification Protein A
Target Information
Gene Symbol AIFM1
Gene Synonyms AIF
AUNX1
CMT2D
CMTX4
COWCK
DFNX5
NADMR
NAMSD
PDCD8
COXPD6
SEMDHL
Gene Full Name apoptosis inducing factor mitochondria associated 1
Gene Summary This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells, and it is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it affects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Mutations in this gene cause combined oxidative phosphorylation deficiency 6 (COXPD6), a severe mitochondrial encephalomyopathy, as well as Cowchock syndrome, also known as X-linked recessive Charcot-Marie-Tooth disease-4 (CMTX-4), a disorder resulting in neuropathy, and axonal and motor-sensory defects with deafness and cognitive disability. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 10. [provided by RefSeq, Aug 2015]
Molecular Weight(MW) 67kDa
Cellular Localization Mitochondrion intermembrane space, Mitochondrion inner membrane, Cytoplasm, Nucleus, Membrane.
Application

WB

25 ug total protein per lane of various lysates (see on figure) probed with AIF monoclonal antibody, unconjugated at 1:1000 dilution and 4°C overnight incubation.Followed by conjugated secondary antibody incubation at r.t. for 60 min.

IHC

Paraformaldehyde-fixed, paraffin embedded Human Liver ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AIF Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by conjugation to the SP Kit and DAB staining.

IF-P

Paraformaldehyde-fixed, paraffin embeddedHuman kidney; Antigen retrieval by boiling insodium citrate buffer (pH6.0) for 15 min; Antibodyincubation with AlF Monoclonal Antibody,Unconjugated at 1:200 overnight at4°C, followed by a BF488 conjugated Goat Anti-Rabbit lgG antibody at 37°Cfor 90 minutes, DAPl (blue) was usedstain the cell nuclei.
Application Notes WB:1:500-1:2000
IHC:1:50-1:200
IF-P:1:50-1:200
Additional Information
Form Liquid
Storage Instructions Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer pH7.4, 150mM NaCl, 0.02% Proclin300 and 50% Glycerol.
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