WB
25 ug total protein per lane of various lysates (see on figure) probed with AIF monoclonal antibody, unconjugated at 1:1000 dilution and 4°C overnight incubation.Followed by conjugated secondary antibody incubation at r.t. for 60 min.
IHC
Paraformaldehyde-fixed, paraffin embedded Human Liver ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AIF Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by conjugation to the SP Kit and DAB staining.
IF-P
Paraformaldehyde-fixed, paraffin embeddedHuman kidney; Antigen retrieval by boiling insodium citrate buffer (pH6.0) for 15 min; Antibodyincubation with AlF Monoclonal Antibody,Unconjugated at 1:200 overnight at4°C, followed by a BF488 conjugated Goat Anti-Rabbit lgG antibody at 37°Cfor 90 minutes, DAPl (blue) was usedstain the cell nuclei.