WB
25 ug total protein per lane of various lysates (see on figure) probed with AIF monoclonal antibody, unconjugated at 1:1000 dilution and 4°C overnight incubation.Followed by conjugated secondary antibody incubation at r.t. for 60 min.IHC
Paraformaldehyde-fixed, paraffin embedded Human Liver ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AIF Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by conjugation to the SP Kit and DAB staining.IF-P
Paraformaldehyde-fixed, paraffin embeddedHuman kidney; Antigen retrieval by boiling insodium citrate buffer (pH6.0) for 15 min; Antibodyincubation with AlF Monoclonal Antibody,Unconjugated at 1:200 overnight at4°C, followed by a BF488 conjugated Goat Anti-Rabbit lgG antibody at 37°Cfor 90 minutes, DAPl (blue) was usedstain the cell nuclei.| Product Name | AIF Recombinant Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthesized peptide derived from human AIF: 510-565 |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-PIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:200 IF-P:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | AIFM1 |
|---|---|
| Gene Synonyms | AIF AUNX1 CMT2D CMTX4 COWCK DFNX5 NADMR NAMSD PDCD8 COXPD6 SEMDHL |
| Gene Full Name | apoptosis inducing factor mitochondria associated 1 |
| Gene Summary | This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells, and it is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it affects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Mutations in this gene cause combined oxidative phosphorylation deficiency 6 (COXPD6), a severe mitochondrial encephalomyopathy, as well as Cowchock syndrome, also known as X-linked recessive Charcot-Marie-Tooth disease-4 (CMTX-4), a disorder resulting in neuropathy, and axonal and motor-sensory defects with deafness and cognitive disability. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 10. [provided by RefSeq, Aug 2015] |
| Molecular Weight(MW) | 67kDa |
| Cellular Localization | Mitochondrion intermembrane space, Mitochondrion inner membrane, Cytoplasm, Nucleus, Membrane. |
WB
25 ug total protein per lane of various lysates (see on figure) probed with AIF monoclonal antibody, unconjugated at 1:1000 dilution and 4°C overnight incubation.Followed by conjugated secondary antibody incubation at r.t. for 60 min.
IHC
Paraformaldehyde-fixed, paraffin embedded Human Liver ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AIF Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by conjugation to the SP Kit and DAB staining.
IF-P
Paraformaldehyde-fixed, paraffin embeddedHuman kidney; Antigen retrieval by boiling insodium citrate buffer (pH6.0) for 15 min; Antibodyincubation with AlF Monoclonal Antibody,Unconjugated at 1:200 overnight at4°C, followed by a BF488 conjugated Goat Anti-Rabbit lgG antibody at 37°Cfor 90 minutes, DAPl (blue) was usedstain the cell nuclei.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:200 IF-P:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | pH7.4, 150mM NaCl, 0.02% Proclin300 and 50% Glycerol. |
Data sheet for OM644109