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Anti-AIF antibody
Catalog Number:OM642047
OM642047
Discount Price
OM642047-100ul
1~2 Week
100ul
OM642047-50ul
50ul
OM642047-20ul
1~2 Week
20ul
1~2 Week
Product Profile
Product Name Anti-AIF antibody
Antibody Type Primary Antibodies
Immunogen Polypeptide
Key Feature
Clonality polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications ELISAICC/IFIHCWB
WB:1:200-1:2000
IHC:1:200-1:1000
ICC/IF:1:100-1:500
Species Reactivity HumanMouseRat
Concentration 1mg/ml
Purification Protein A
Target Information
Gene Symbol AIFM1
Gene Synonyms AIFM1; AIF
AUNX1
CMT2D
CMTX4
COWCK
DFNX5
NADMR
NAMSD
PDCD8
COXPD6
SEMDHL
Gene Full Name apoptosis inducing factor mitochondria associated 1
Gene Summary This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells, and it is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it affects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Mutations in this gene cause combined oxidative phosphorylation deficiency 6 (COXPD6), a severe mitochondrial encephalomyopathy, as well as Cowchock syndrome, also known as X-linked recessive Charcot-Marie-Tooth disease-4 (CMTX-4), a disorder resulting in neuropathy, and axonal and motor-sensory defects with deafness and cognitive disability. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 10. [provided by RefSeq, Aug 2015]
Molecular Weight(MW) 67 KDa
Source Rabbit
Cellular Localization Mitochondrion intermembrane space. Mitochondrion inner membrane. Cytoplasm. Nucleus.
Application

WB

Western blot analysis using AIF antibody against A549 (1), Raji (2), HUVEC (3) cell lysate.12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.

IHC

Immunohistochemical analysis of paraffin-embedded breast cancer tissues using AIF antibody with DAB staining.Pre-treat the sections with heat-mediated antigen retrieval using sodium citrate buffer (pH 6.0) (OM750020) for 2 minutes. Wash the sections with ddH₂O and PBS (OM750003). Block the tissue with 10% non-immune goat serum(OM760028) at room temperature for 30 minutes. Incubate the tissue with the primary antibody diluted at a ratio of 1:1500 at 4°C overnight. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:200 and incubate for one hour. Use DAB(OM760029)as the chromogenic agent. Counterstain the tissue with hematoxylin, and mount the tissue sections with neutral gum.

ICC/IF

Immunofluorescence analysis of Hela cells using AIF antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Omnimabs® 594-Phalloidin.Cells are fixed in 4% paraformaldehyde at room temperature for 20 minutes. Then, they are permeabilized with a PBS (OM750003) solution containing 0.1% Triton X-100(OM750021) at room temperature for 15 minutes. Subsequently, the cells are blocked with 10% non - immune goat serum(OM760028) at room temperature for 1 hour.The cells are incubated overnight at 4°C with the primary antibody diluted 1:100 in PBS. The secondary antibody, Omnimabs® 488 Goat Ant-Rabbit IgG(H&L) (Green,OM643486), is diluted at a ratio of 1:400 and incubated with the cells for 1 hour.Nuclear DNA is labeled with DAPI (Blue,OM643160). F-actin is stained with Omnimabs® 594-Phalloidin (Red,OM750007) diluted 1:100 for 30 minutes.
Application Notes WB:1:200-1:2000
IHC:1:200-1:1000
ICC/IF:1:100-1:500
Additional Information
Form Liquid
Storage Instructions Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer Purified antibody in PBS with 0.05% sodium azide.
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