WB
Western blot analysis of extracts from Pc12, using DPP9 - Catalytic domain Antibody. The lane on the left was treated with blocking peptide.IHC
DPP9 - Catalytic domain Antibody at 1/100 staining Rat heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.ICC/IF
DPP9 - Catalytic domain Antibody staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(1:200) and mouse anti-beta tubulin Ab(1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue).| Product Name | Rabbit polyclonal antibody to DPP9 - Catalytic domain |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthesized peptide derived from human DPP9(Accession Q86TI2), corresponding to amino acid residues V280-I330. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:200 ICC/IF:1:100-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DPP9 |
|---|---|
| Gene Synonyms | DP9 DPLP9 DPRP2 HATIS DPP IX DPRP-2 IMD111 |
| Gene Full Name | dipeptidyl peptidase 9 |
| Gene Summary | This gene encodes a protein that is a member of the S9B family in clan SC of the serine proteases. The protein has been shown to have post-proline dipeptidyl aminopeptidase activity, cleaving Xaa-Pro dipeptides from the N-termini of proteins. Although the activity of this protein is similar to that of dipeptidyl peptidase 4 (DPP4), it does not appear to be membrane bound. In general, dipeptidyl peptidases appear to be involved in the regulation of the activity of their substrates and have been linked to a variety of diseases including type 2 diabetes, obesity and cancer. Several transcript variants of this gene have been described but not fully characterized. [provided by RefSeq, Jul 2008] |
| Alternative Names | Dipeptidyl peptidase 9 Dipeptidyl peptidase IV related protein 2 Dipeptidyl peptidase IV-related protein 2 Dipeptidyl peptidase IX Dipeptidyl peptidase like protein 9 Dipeptidyl peptidase-like protein 9 Dipeptidylpeptidase 9 Dipeptidylpeptidase IX DKFZp762F117 DP 9 DP9 DPLP 9 DPLP9 DPP 9 DPP IX DPP9 DPP9_HUMAN DPRP 2 DPRP-2 DPRP2 FLJ16073 |
| Molecular Weight(MW) | 98kDa |
| Cellular Localization | Cytoplasm. Nucleus. |
WB
Western blot analysis of extracts from Pc12, using DPP9 - Catalytic domain Antibody. The lane on the left was treated with blocking peptide.
IHC
DPP9 - Catalytic domain Antibody at 1/100 staining Rat heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.
ICC/IF
DPP9 - Catalytic domain Antibody staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(1:200) and mouse anti-beta tubulin Ab(1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue).| Application Notes | WB:1:500-1:2000 IHC:1:50-1:200 ICC/IF:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C. Stable for 12 months from date of receipt. |
| Storage Buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
