WB
Western blot analysis of p38 alpha/MAPK14 + p38 beta/MAPK11 + p38 gamma/MAPK12 on different lysates with Rabbit anti-p38 alpha/MAPK14 + p38 beta/MAPK11 + p38 gamma/MAPK12 antibody at 1/2,000 dilution. Lane 1: 293T cell lysate (30 µg/Lane), Lane 2: HeLa cell lysate (30 µg/Lane), Lane 3: K-562 cell lysate (30 µg/Lane), Lane 4: MDA-MB-231 cell lysate (30 µg/Lane), Lane 5: COS-1 cell lysate (30 µg/Lane), Lane 6: Neuro-2a cell lysate (30 µg/Lane), Lane 7: PC-12 cell lysate (30 µg/Lane), Lane 8: Mouse skeletal muscle tissue lysate (30 µg/Lane), Lane 9: Rat skeletal muscle tissue lysate (30 µg/Lane), Lane 10: Rat heart tissue lysate (30 µg/Lane), Exposure time: 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.WB
Western blot analysis of p38 alpha/MAPK14 + p38 beta/MAPK11 + p38 gamma/MAPK12 with Rabbit anti-p38 alpha/MAPK14 + p38 beta/MAPK11 + p38 gamma/MAPK12 antibody at 1/2,000 dilution. Lane 1: Human p38 gamma recombinant protein, 30ng/Lane, Lane 2: Human p38 alpha recombinant protein, 50ng/Lane, Lane 3: Human p38 delta recombinant protein, 50ng/Lane, Lane 4: Human p38 beta recombinant protein, 50ng/Lane, Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.| Product Name | p38 alpha/MAPK14 + p38 beta/MAPK11 + p38 gamma/MAPK12 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human MAPK12 aa 1-367 / 367. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | MAPK11,MAPK12,MAPK14 |
|---|---|
| Gene Full Name | mitogen-activated protein kinase 11/12/14 |
| Alternative Names | ERK 6 antibody ERK-6 antibody ERK6 antibody Extracellular signal-regulated kinase 6 antibody MAP kinase 12 antibody MAP kinase p38 gamma antibody MAPK 12 antibody Mapk12 antibody Mitogen Activated Protein Kinase 12 antibody Mitogen activated protein kinase p38 gamma antibody ERK 6 antibody ERK-6 antibody ERK6 antibody Extracellular signal-regulated kinase 6 antibody MAP kinase 12 antibody MAP kinase p38 gamma antibody MAPK 12 antibody Mapk12 antibody Mitogen Activated Protein Kinase 12 antibody Mitogen activated protein kinase p38 gamma antibody Mitogen-activated protein kinase 12 antibody Mitogen-activated protein kinase p38 gamma antibody MK12_HUMAN antibody P38 GAMMA antibody P38GAMMA antibody PRKM12 antibody SAPK 3 antibody SAPK-3 antibody SAPK3 antibody Stress Activated Protein Kinase 3 antibody Stress-activated protein kinase 3 antibody |
| Molecular Weight(MW) | 42kDa |
| Function | A mitogen-activated protein kinase (MAPK or MAP kinase) is a type of protein kinase that is specific to the amino acids serine and threonine (i.e., a serine/threonine-specific protein kinase). MAPKs are involved in directing cellular responses to a diverse array of stimuli, such as mitogens, osmotic stress, heat shock and proinflammatory cytokines. They regulate cell functions including proliferation, gene expression, differentiation, mitosis, cell survival, and apoptosis. MAP kinases are found in eukaryotes only, but they are fairly diverse and encountered in all animals, fungi and plants, and even in an array of unicellular eukaryotes. MAPKs belong to the CMGC (CDK/MAPK/GSK3/CLK) kinase group. The closest relatives of MAPKs are the cyclin-dependent kinases (CDKs). |
| Cellular Localization | Cytoplasm, Nucleus, Mitochondrion. |
WB
Western blot analysis of p38 alpha/MAPK14 + p38 beta/MAPK11 + p38 gamma/MAPK12 on different lysates with Rabbit anti-p38 alpha/MAPK14 + p38 beta/MAPK11 + p38 gamma/MAPK12 antibody at 1/2,000 dilution. Lane 1: 293T cell lysate (30 µg/Lane), Lane 2: HeLa cell lysate (30 µg/Lane), Lane 3: K-562 cell lysate (30 µg/Lane), Lane 4: MDA-MB-231 cell lysate (30 µg/Lane), Lane 5: COS-1 cell lysate (30 µg/Lane), Lane 6: Neuro-2a cell lysate (30 µg/Lane), Lane 7: PC-12 cell lysate (30 µg/Lane), Lane 8: Mouse skeletal muscle tissue lysate (30 µg/Lane), Lane 9: Rat skeletal muscle tissue lysate (30 µg/Lane), Lane 10: Rat heart tissue lysate (30 µg/Lane), Exposure time: 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
WB
Western blot analysis of p38 alpha/MAPK14 + p38 beta/MAPK11 + p38 gamma/MAPK12 with Rabbit anti-p38 alpha/MAPK14 + p38 beta/MAPK11 + p38 gamma/MAPK12 antibody at 1/2,000 dilution. Lane 1: Human p38 gamma recombinant protein, 30ng/Lane, Lane 2: Human p38 alpha recombinant protein, 50ng/Lane, Lane 3: Human p38 delta recombinant protein, 50ng/Lane, Lane 4: Human p38 beta recombinant protein, 50ng/Lane, Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.| Application Notes | WB:1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
